1,755 results

1. pJF6

   Plasmid

   #139040

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   gfp (includes STOP codon, PATC introns)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH4542

   Plasmid

   #200779

   Purpose

   Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GCaMP6
   

   Use

   Tags

   mNeptune

   Expression

   Worm

   Mutation

   Promoter

   Ptwk-40

   Available since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKS235

   Plasmid

   #191855

   Purpose

   Negative selection marker for MosSCI

   Depositor

   K Subramaniam

   Article

   Revathi et al MicroPubl Biol. 2022 Oct 20;2022:10.1791

   Insert

   peel-1 toxic protein for negative selection against extrachromosomal array
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.41

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BSP605

   Plasmid

   #122250

   Purpose

   expression vector for mCerulean under EFT3 promoter

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   mCerulean
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   EFT3

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PU6::klp-12_sgRNA

   Plasmid

   #46170

   Purpose

   klp-12 targeting sgRNA

   Depositor

   John Calarco

   Article

   Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

   Insert

   klp-12 targeting sgRNA (klp-12 Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. ChrII_ttTi5605_loxP_myo2_neoR

   Plasmid

   #194056

   Purpose

   Dual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   ttTi5605
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

   Plasmid

   #66753

   Purpose

   miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

   Depositor

   Yishi Jin

   Article

   Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   miniSOG
   

   Use

   Tags

   tomm-20 (N terminal 55 aa)

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1-gpa-2P::his-24::Cerulean

   Plasmid

   #124337

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   gpa-2, Cerulean (gpa-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAB16 (pmyo-3::QuasAr)

    Plasmid

    #130272

    Purpose

    Expresses the voltage sensor QuasAr in BWMs of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

    Insert

    QuasAr
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW2341

    Plasmid

    #186950

    Purpose

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker

    Depositor

    Jordan Ward

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ChrV_oxTi365_loxP_myo2_neoR

    Plasmid

    #194057

    Purpose

    Dual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    oxTi365
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    None

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMS158

    Plasmid

    #215679

    Purpose

    Empty repair plasmid for ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

    Plasmid

    #159813

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRB1080

    Plasmid

    #71309

    Purpose

    Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

    Depositor

    Andrew Fire

    Article

    Bell et al Genetics. 2015 Dec 17. pii: genetics.115

    Insert

    VQR Cas9 variant
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159850

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

    Plasmid

    #200367

    Purpose

    cbr-unc-119 rescue gene for use as co-injection marker.

    Depositor

    Christian Froekjaer-Jensen

    Article

    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

    Insert

    [cbr-unc-119(+)]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJH127

    Plasmid

    #162483

    Purpose

    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

    Depositor

    Bob Goldstein

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    

    Use

    CRISPR

    Tags

    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge