1,779 results

1. SP6-sgRNA-scaffold

   Plasmid

   #47912

   Purpose

   Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   sgRNA 3' end

   Expression

   Worm

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH4542

   Plasmid

   #200779

   Purpose

   Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

   Depositor

   Mei Zhen

   Article

   Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

   Insert

   GCaMP6
   

   Tags

   mNeptune

   Expression

   Worm

   Promoter

   Ptwk-40

   Available Since

   May 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJA304

   Plasmid

   #28091

   Depositor

   Julie Ahringer

   Article

   Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

   Insert

   (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
   

   Expression

   Worm

   Available Since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ1662

   Plasmid

   #51482

   Purpose

   miniMos transposable element with HygroR, MCS cloning

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

   Plasmid

   #159799

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS1-gpa-2P::his-24::Cerulean

   Plasmid

   #124337

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   gpa-2, Cerulean (gpa-2 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGC185

   Plasmid

   #19624

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
   

   Use

   Flp/frt

   Expression

   Worm

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHygCG2r

   Plasmid

   #200074

   Purpose

   hygR selection mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Tags

   RFP

   Expression

   Worm

   Promoter

   Pacr-2(s)

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHW581

    Plasmid

    #198818

    Purpose

    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFJ210

    Plasmid

    #30538

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Expression

    Worm

    Available Since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSN444

    Plasmid

    #108331

    Purpose

    Expresses Sat2 for Nourseothricin resistance selection of C. elegans

    Depositor

    Shinsuke Niwa

    Article

    Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

    Insert

    Streptothricin acetyl transferase 2
    

    Expression

    Worm

    Promoter

    rps-27 promoter

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNM3732

    Plasmid

    #153086

    Purpose

    mec-4p GFP-C1 positive control for RMCE integration

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p GFP-C1 tbb-2 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TC476

    Plasmid

    #122629

    Purpose

    Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
    

    Expression

    Worm

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEY51

    Plasmid

    #191054

    Purpose

    mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    mec-3 (mec-3 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEY26

    Plasmid

    #191031

    Purpose

    cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    cho-1(3.3-2.6k) (cho-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJW2072

    Plasmid

    #154334

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEY52

    Plasmid

    #191055

    Purpose

    odr-1 fluorescent neural reporter driving nuclear mNeptune2.5 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    odr-1 (odr-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS1-lim-4P::his-24::Cerulean

    Plasmid

    #124336

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    lim-4, Cerulean (lim-4 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge