1,748 results

1. pEft-3::oxCerulean-oxVenus::Lgg-1

   Plasmid

   #68040

   Purpose

   Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagy

   Depositor

   Dana Miller

   Article

   Chapin et al Aging (Albany NY). 2015 Jun 30.

   Insert

   pEft-3::oxCerulean-oxVenus::Lgg-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

   Plasmid

   #129538

   Purpose

   modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

   Depositor

   Arneet Saltzman

   Article

   Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox; Mos1-mediated single copy insertion (mos…

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

   Plasmid

   #168170

   Purpose

   Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

   Depositor

   Alexander Gottschalk

   Article

   Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

   Insert

   YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   E497K and C566D

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

   Plasmid

   #108362

   Purpose

   Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

   Depositor

   Luisa Cochella

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Inserts

   Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
   mCherry
   his-15 (his-15 Nematode)
   unc-54 3' UTR
   

   Use

   Tags

   2xFlag, MYC, T2A peptide, and TY1 linker

   Expression

   Worm

   Mutation

   Promoter

   A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

   Available since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJH3377

   Plasmid

   #175286

   Purpose

   Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms d/f of DAF-16

   Depositor

   Mei Zhen

   Article

   Hung et al Development. 2014 Apr;141(8):1767-79. do

   Insert

   daf-16 d/f (daf-16 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pges-1

   Available since

   Nov. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEY12

   Plasmid

   #191018

   Purpose

   Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   rab-3 (rab-3 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. punc-119cR

   Plasmid

   #21596

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119:mCherry (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   mCherry

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. XW52

    Plasmid

    #65838

    Purpose

    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Czipper-QF-AD-NLS
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Pmig-13

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEY46

    Plasmid

    #191050

    Purpose

    glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    glr-1 (glr-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEY38

    Plasmid

    #191042

    Purpose

    unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    unc-8 (unc-8 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEY13

    Plasmid

    #191019

    Purpose

    Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    arrd-4 (ZK938.2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEY20

    Plasmid

    #191026

    Purpose

    gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    gcy-18 (gcy-18 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW1838

    Plasmid

    #154324

    Purpose

    sgRNA Destination Vector for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD_Psra-6::ChR2(H134R)::mCherry

    Plasmid

    #117419

    Purpose

    For selectively expressing ChR2(H134R) in ASH sensory neurons under the expression of a Psra-6 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    sra-6

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDR3.10

    Plasmid

    #19367

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    msp-142 ORF+3' in pDONR201
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMLS328

    Plasmid

    #73717

    Purpose

    C. elegans germline CRE expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-CRE
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ2359 - intron GG2 - 250bp

    Plasmid

    #159878

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG2 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge