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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Article

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEG476

Plasmid

#40198

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (S404A) (mex-5 Nematode)

Tags

Dendra2 PAFP (sequence optimized for worm express…

Expression

Worm

Mutation

S404A

Promoter

pie-1

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
TU#711

Plasmid

#15279

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY20

Plasmid

#191026

Purpose

gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-18 (gcy-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pmyo-3

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

Plasmid

#108362

Purpose

Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

Depositor

Luisa Cochella

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Inserts

Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
mCherry
his-15 (his-15 Nematode)
unc-54 3' UTR

Tags

2xFlag, MYC, T2A peptide, and TY1 linker

Expression

Worm

Promoter

A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJRK249

Plasmid

#173747

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR

Expression

Worm

Promoter

Prps-0

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGC381

Plasmid

#19663

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<*-CFP::let-858(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

Plasmid

#66781

Purpose

Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Mutation

G426C

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Article

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pnmr-1

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pIN4_tsp-6_mScarlet

Plasmid

#182935

Purpose

Expresses tsp-6::mScarlet under its native promoter (amphid and phasmid ciliated neurons in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

Ptsp-6-3::tsp-6::flexible linker::mScarlet (C. elegans, genomic)

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Promoter

tsp-6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSR40

Plasmid

#69257

Purpose

plin-31::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

lin-31

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

Plasmid

#129538

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2

Plasmid

#182359

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Ultra-pan-neuronal positive control pUNP::gfp(NLS)

Expression

Worm

Promoter

Promoter Ultra Pan Neuronal

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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