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pAS1-gpa-2P::his-24::Cerulean

Plasmid

#124337

Purpose

Multicolor Worms for Neuronal ID

Depositor

Andrew Leifer

Insert

gpa-2, Cerulean (gpa-2 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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BSP606

Plasmid

#122251

Purpose

expression vector for mECitrine under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mECitrine

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BSP611

Plasmid

#122256

Purpose

expression vector for mtagRFP-T under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mtagRFP-T

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGC680

Plasmid

#107018

Purpose

GFP reporter of lag-2 driven by 3 kb promoter

Depositor

Jane Hubbard

Publication

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Tags

GFP

Expression

Worm

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGM59

Plasmid

#74785

Purpose

Expresses 3XFLAG::GR LBD::DAF-16A from the hsp16.48 promoter

Depositor

Jordan Ward, Keith Yamamoto

Publication

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Insert

3X FLAG::GR LBD::DAF-16A (daf-16, NR3C1 Synthetic, Nematode, Human)

Expression

Worm

Promoter

hsp16.48

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pNP160

Plasmid

#106362

Purpose

To insert a single copy of a construct at a site on Chr II, corresponding to RDE-1 expressed specifically in the intestine, for tissue-specific RNAi.

Depositor

Jonathan Ewbank

Insert

ttTi5605_SEC_mtl-2p_RDE-1_3'rde-1

Expression

Worm

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BSP188

Plasmid

#110917

Purpose

For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

unc54 Terminator

Use

Synthetic Biology; Yeast homologous recombination…

Expression

Worm

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Publication

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Tags

mito::GCaMP3

Expression

Worm

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS235

Plasmid

#73683

Purpose

SapTrap 3-site Destination vector for C-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)

Plasmid

#107134

Purpose

cGAL-N driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBN258

Plasmid

#86721

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Punc-119::FLP D5

Expression

Worm

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCZGY2844

Plasmid

#87760

Purpose

Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.

Depositor

Andrew Chisholm

Publication

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Punc-17b-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Punc-17b

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBN40

Plasmid

#86715

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Plmn-1::gfp::his-58

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN263

Plasmid

#86729

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-2::FLP D5

Expression

Worm

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBN8

Plasmid

#86711

Purpose

Cloning plasmid for MosSCI transgenesis in C. elegans

Depositor

Peter Askjaer

Publication

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Type

Empty backbone

Expression

Worm

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBN9

Plasmid

#86712

Purpose

Cloning plasmid for MosSCI transgenesis in C. elegans

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Type

Empty backbone

Expression

Worm

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ150-cul4

Plasmid

#80353

Purpose

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Publication

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::cul-4 3'UTR

Expression

Worm

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJL1703

Plasmid

#78196

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

klp-6

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only