1,748 results

1. pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)

   Plasmid

   #159889

   Purpose

   Three-fragment Gateway compatible promoter for expression in all muscles of C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   [4-1] - Promoter - Pmlc-2(no_start)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

   Plasmid

   #159794

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

   Plasmid

   #159825

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEM266 - pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

   Plasmid

   #159829

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pEXP(mosTI, hygroR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSEM267 - pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

   Plasmid

   #159833

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pEXP(mosTI, neoR)[Pmex-5 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

   Plasmid

   #159836

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI Pmlc-2_GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHA841 - hrp-1p::hrp-1HsLCWT

   Plasmid

   #139198

   Purpose

   Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   last exon replaced with correspoding human sequen…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHA842 - hrp-1p::hrp-1HsLCD290V

   Plasmid

   #139199

   Purpose

   Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   D290V, last exon replaced with correspoding human…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHA847 - mec-4p::hrp-1mScarlet

    Plasmid

    #139201

    Purpose

    Expresses mScarlet tagged hrp-1 in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHA848 - mec-4p::hrp-1HsLCWTmScarlet

    Plasmid

    #139202

    Purpose

    Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, last exon rep…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139203

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHA844 - hrp-1p::hrp-1mScarlet

    Plasmid

    #139204

    Purpose

    Expresses mScarlet tagged hrp-1

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

    Plasmid

    #139205

    Purpose

    Expresses mScarlet tagged hrp-1HsLCWT

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, last exon rep…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139206

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHA850 - osm-10p::FynY531F

    Plasmid

    #139207

    Purpose

    Express activated Fyn kinase in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHA851 - osm-10p::Fynempty

    Plasmid

    #139208

    Purpose

    Control vector for Fyn kinase expression in osm-10 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    deletion and frame shift to prevent Fyn expression

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHA852 - mec-4p::FynY531F

    Plasmid

    #139209

    Purpose

    Expresses activated Fyn kinase in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    Fyn (FYN Human)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Y531F

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid

    #159838

    Purpose

    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHJ4

    Plasmid

    #158785

    Purpose

    Pan-neuronal nuclear-localized GCaMP expression in C. elegans

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    Prab-3::nls::GCaMP6m
    

    Use

    Tags

    NLS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJW1884

    Plasmid

    #154332

    Purpose

    sgRNA Target Vector for CRISPR

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJW1827

    Plasmid

    #154323

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJW1841

    Plasmid

    #154338

    Purpose

    Promoter reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJW1948

    Plasmid

    #154340

    Purpose

    Enhancer reporter

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDD289

    Plasmid

    #137018

    Purpose

    GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    GFP-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDD376

    Plasmid

    #137022

    Purpose

    SapTrap mKate2-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mKate2
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mKate2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXF90

    Plasmid

    #139044

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag::HDEL (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCZGY921

    Plasmid

    #135092

    Purpose

    Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Use

    Tags

    GFP and Synthetic Intron

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCZGY2750

    Plasmid

    #135094

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr IV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    sgRNA for cxTi10082 (actgttggatgcctgtgtag)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJPL3[flp-6p::cmk-1::GFP]

    Plasmid

    #124613

    Purpose

    Used to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons

    Depositor

    Anne Brunet

    Article

    Lim et al J Neurosci. 2018 Jul 4;38(27):6114-6129.

    Insert

    [flp-6p::cmk-1::GFP]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge