7,942 results

1. pAHYB-GFP

   Plasmid

   #85074

   Purpose

   Expression of GFP in Pichia pastoris

   Depositor

   Sheng Yang

   Article

   Yang et al Yeast. 2014 Apr;31(4):115-25.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENO1-BFP-NAT

   Plasmid

   #50046

   Purpose

   Expression of BFP in Candida albicans

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

   Insert

   BFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pENO1

   Available since

   Jan. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CUP1-∆ssCPY*-mGFP in pRS315

   Plasmid

   #221158

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mGFP

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CUP1-∆ssCPY*-mScarletI in pRS315

   Plasmid

   #221159

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Use

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniTurbo-V5_pRS415

   Plasmid

   #107168

   Purpose

   expresses V5-tagged miniTurbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML107

   Plasmid

   #67639

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pSNR52 and pTDH3 (aka GAP promoter)

   Available since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #49425

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

   Depositor

   Daniel Klionsky

   Article

   Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB5319

    Plasmid

    #204829

    Purpose

    An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetSW1 promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yoTagRFP-T-Kan

    Plasmid

    #44906

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESC-leu2d(empty)

    Plasmid

    #20120

    Depositor

    Jay Keasling

    Article

    Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

    Plasmid

    #198417

    Purpose

    Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMetYC-Dest

    Plasmid

    #105081

    Purpose

    Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    Cub-PLV

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR196

    Plasmid

    #36029

    Depositor

    Dominique Loque, Doris Rentsch, Wolf Frommer

    Article

    Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. p415-PtrpC-Cas9-TtrpC-CYC1t

    Plasmid

    #68059

    Purpose

    Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    humanized Cas9
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2015-N iFAST

    Plasmid

    #209415

    Purpose

    iFAST expression plasmid using MET3p

    Depositor

    Patrick Van Dijck

    Article

    Devos et al Microbiology (Reading). 2024 Mar;170(3).

    Insert

    iFAST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET3

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MS2-Snapf

    Plasmid

    #65860

    Purpose

    Expresses RNA-binding protein MS2 with fast SNAP fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    Fast SNAP
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBEVY-GL

    Plasmid

    #51225

    Purpose

    bi-directional expression vector for yeast, galactose inducible, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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