8,058 results

1. pBS-SKII-3XHA-fSNAP-Kan

   Plasmid

   #188918

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-Kan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDBD-M

   Plasmid

   #50572

   Purpose

   Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

   Depositor

   Alexander Varshavsky

   Article

   Turner et al Science. 2000 Sep 22;289(5487):2117-20.

   Insert

   DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pOAD

   Plasmid

   #196825

   Purpose

   Yeast two-hybrid ; Gal4 activation domain

   Depositor

   Stan Fields

   Article

   Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

   Type

   Empty backbone

   Use

   Tags

   Gal4 Activation domain

   Expression

   Yeast

   Mutation

   Promoter

   ADH

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFP5EF3

   Plasmid

   #185881

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB8380

   Plasmid

   #126914

   Purpose

   Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS424-TUB2-Cterminal6xHis

   Plasmid

   #60386

   Purpose

   Expression of yeast beta tubulin (TUB2)-Cterminal 6xHis using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   6xHIS

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCT-4M5.3

   Plasmid

   #41844

   Depositor

   Dane Wittrup

   Article

   Midelfort et al J Mol Biol. 2004 Oct 22;343(3):685-701.

   Insert

   4M5.3
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKmK.T3

   Plasmid

   #125055

   Purpose

   Storage plasmid for K.marxianus terminator; type 4 part

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   KMXK_A3020 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS414 GPD-Sis1

   Plasmid

   #18687

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Sis1 (SIS1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER

    Plasmid

    #52687

    Purpose

    Same as 41025, with additional linker

    Depositor

    Michael Nick Boddy

    Insert

    tetO7-Pcyc1-GFP+LINKER
    

    Use

    Promoter replacement

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP4M

    Plasmid

    #104508

    Purpose

    Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

    Depositor

    Claudia Vickers

    Article

    Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

    Insert

    SeGAL2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-link-yoLSS-mKate2-CaURA3

    Plasmid

    #44886

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAT-TET-OFF -MET - 3xHA

    Plasmid

    #182526

    Purpose

    Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYC61

    Plasmid

    #63925

    Purpose

    Recipient replicative vector to construct CFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC55

    Plasmid

    #63922

    Purpose

    Recipient replicative vector to construct YFP tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYC42

    Plasmid

    #63920

    Purpose

    Recipient replicative vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKT0175

    Plasmid

    #8733

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECitrine
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with S65G, V68L, Q69M, S72A, T203Y

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES2-gRNA-hyg-MCS

    Plasmid

    #107734

    Purpose

    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.

    Depositor

    Sheng Yang

    Article

    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

    Insert

    X-3 site gRNA expression cassettes
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52p

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOP462

    Plasmid

    #85935

    Purpose

    Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 13C6 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICE TRP1

    Plasmid

    #122881

    Purpose

    TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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