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pJRoC30_VP11H

Plasmid

#188063

Purpose

PROSS-designed versatile peroxidase 11H

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-HaloTag-DRS2

Plasmid

#177103

Purpose

Expresses HaloTag-Drs2 in yeast cells

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

DRS2 (DRS2 Budding Yeast)

Expression

Yeast

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

Plasmid

#179079

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Publication

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Expression

Yeast

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Publication

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG

Plasmid

#176400

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

FRB-RGG-GFP-RGG-RGG

Tags

FRB and GFP

Expression

Yeast

Promoter

GAL1

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

Plasmid

#179078

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179064

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VAM3

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)

Plasmid

#179054

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubules

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-TUB1

Expression

Yeast

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Publication

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Expression

Yeast

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK093

Plasmid

#171365

Purpose

Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGRCRE

Plasmid

#129116

Purpose

Expresses cre recombinase in Z. rouxii yeast cells

Depositor

Paolo Giudici

Publication

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Insert

cre

Use

Cre/Lox

Expression

Yeast

Promoter

S. cerevisiae GAL1 promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42H_PTEF1_dCas9_TCYC1

Plasmid

#177155

Purpose

Expresses dCas9 in yeast cells

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

Nuclease-null Cas9

Tags

SV40 NLS

Expression

Yeast

Promoter

TEF1 promoter

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI2

Plasmid

#176156

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Publication

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_14_GlyOx

Plasmid

#177711

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Publication

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRS423-mitoT

Plasmid

#174525

Purpose

Expresses yeast variant of mitoT synthetic intermembrane tether bridging the inner and outer mitochondrial membranes

Depositor

Oleh Khalimonchuk

Publication

Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

Insert

Yeast mitochondrial intermembrane tether

Use

Synthetic Biology

Tags

eGFP

Expression

Yeast

Promoter

MET25

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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