8,418 results
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Plasmid#172188PurposeGenome editing of C. albicansDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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EZ-L175
Plasmid#173025PurposeEncodes OptoAMP1 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L439
Plasmid#169146PurposeEncodes OptoINVRT7 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorPublicationInsertenhanced green fluorescent protein
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cyterm-ymtq2 pDRF1-GW
Plasmid#168083PurposeExpresses Cyterm-ymTq2 to check for monomericity.DepositorInsertcyterm-ymTq2
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarletI pDRF1-GW
Plasmid#168085PurposeExpresses Lifeact-mScarletI to visualize actin in yeastDepositorInsertLifeAct-mScarletI
ExpressionYeastAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNA0304
Plasmid#165612PurposeVector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)DepositorInsertSNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
UseCRISPRExpressionYeastMutationNotI site replaced the CAN1 spacer in parent vect…PromoterSNR52Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGY416
Plasmid#166660PurposeFor light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterGAL1Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-GFP-Dcp2
Plasmid#163585Purposeexpress N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiaeDepositorAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-GFP
Plasmid#166836PurposeExpresses Vac8-GFP in yeasts.DepositorAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-msGFP2-HDEL
Plasmid#160468PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signalDepositorInsertKAR2-msGFP2-HDEL
ExpressionYeastAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
ExpressionYeastPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneExpressionYeastAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
ExpressionYeastAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionYeastAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only