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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBEVY-pT231 scFv-GFP

Plasmid

#218827

Purpose

Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

Depositor

Yongku Cho

Article

Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

Insert

pT231 tau scFv

Expression

Yeast

Promoter

GAL1-10

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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HP-Cas9hGem-Ade2-LEU2-LINEAR

Plasmid

#174839

Purpose

pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TDH3p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBD-PYR1_star-MANDI

Plasmid

#241280

Purpose

Yeast two hybrid vector expressing BD-PYR1_star-MANDI

Depositor

Sean Cutler

Article

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

PYR1_star_mandi (PYR1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMetYC-Dest

Plasmid

#105081

Purpose

Yeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

Cub-PLV

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Tags

NubG

Expression

Yeast

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pV1093:SpRY

Plasmid

#172188

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

GPD

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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