7,933 results

1. pDRF1 yeBFP2-T2A-mCherry

   Plasmid

   #219851

   Purpose

   equimolar expression of yeBFP2 and mCherry in budding yeast

   Depositor

   Bas Teusink

   Insert

   yeBFP2-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDRF1-GW yomTagBFP2-T2A-mCherry

   Plasmid

   #205493

   Purpose

   Expression of yomTagBFP2-T2A-mCherry in yeast

   Depositor

   Bas Teusink

   Insert

   yomTagBFP2-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGBK CUP1 Empty

   Plasmid

   #169710

   Purpose

   Yeast two-hybrid vector using a copper inducible CUP1 promoter

   Depositor

   Bruce Hamilton

   Article

   Hernandez et al bioRxiv

   Type

   Empty backbone

   Use

   Tags

   GAL4 DNA Binding Domain and Myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   CUP1

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRF1-GW yomCherry-T2A-mTurquoise2

   Plasmid

   #118447

   Purpose

   Produces equimolar expression of yeast codon-optimized mCherry and mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   yomCherry-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pI1-MTU-DO-HPH

   Plasmid

   #160014

   Purpose

   Integrative vector for K. marxianus, targeting the LAC4 locus. Hygromycin selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS424GFP-2xPH(Osh2)

   Plasmid

   #36095

   Depositor

   Scott Emr

   Article

   Stefan et al Cell. 2011 Feb 4;144(3):389-401.

   Insert

   2xPH(Osh2) fusion
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PRC1

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal1/10p CAS9 Gal1/10p I-SceI

   Plasmid

   #182519

   Purpose

   Galactose-Induced Expression vector for Cas9 and I-SceI.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Inserts

   I-SceI
   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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