7,935 results

1. pMJ45: pRS426-His-MBP-SpuFz1_Native

   Plasmid

   #205252

   Purpose

   S. cerevisiae expression vector for SpuFz1 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG426GAL-EGFP-ccdB

   Plasmid

   #14323

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. THI VP64 pALD4 T-

   Plasmid

   #161481

   Purpose

   Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2-VP64
   gRNA (Non targeting control NTC)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pALD4, pPOR1, and pTEF2

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZJOM10

   Plasmid

   #133639

   Purpose

   Integration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-Vps4-GFP (VPS4 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416 GAL1pro-YFP

   Plasmid

   #224875

   Purpose

   Low copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)

   Depositor

   Paul Herman

   Type

   Empty backbone

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-6xGLY-FLAG-hphMX4

   Plasmid

   #20752

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pROS11

   Plasmid

   #107925

   Purpose

   amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYM29-BirA*

   Plasmid

   #160291

   Purpose

   Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1

   Depositor

   Martin Ott

   Article

   Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

   Insert

   BirA*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCTcon2-5INS_16

   Plasmid

   #102292

   Purpose

   Yeast Surface Display of 5Ins_16 scFV

   Depositor

   Sarel Fleishman

   Article

   Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

   Insert

   Insulin binding scFV
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAWH-largeT

    Plasmid

    #133895

    Purpose

    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LargeT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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