8,173 results
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Plasmid#64511PurposeExpresses destabilized Ametrine in yeast.DepositorInsertAmetrine-ADH1-tail
ExpressionYeastPromoterTDH3Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS34
Plasmid#83796PurposeUseful for tagging yeast proteins with C-terminal mCherry tag; G418 selectionDepositorInsertmCherry
TagsmCherryExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
Tags6XHisExpressionYeastMutationPlease see depositor commentsAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p316CUP1-3SGFP.SG
Plasmid#1207DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo
Plasmid#115420PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
ExpressionYeastPromoterTPI1Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-EGFP-ccdB
Plasmid#14319DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL5-mCherry2Bx6
Plasmid#105253PurposePlasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GTT-g
Plasmid#81109PurposeGene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertsfGFP
UseCre/LoxTagssfGFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -MET - 3xHA
Plasmid#182526PurposeDoxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.DepositorInsert7 Copies of TetO Box
Tags3HAExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pV1329
Plasmid#111438PurposeSolo vector pV1326 +sgCgADE2DepositorInsertCaCas9/sgCgADE2
UseCRISPRExpressionYeastAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSc-His6-Sem1Sc
Plasmid#83235PurposepGREG600-His6-Sem1Sc, Expresses the His6-Sem1 in yeastDepositorAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDBD-R
Plasmid#50571PurposeDouble Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572DepositorInsertDHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
ExpressionYeastMutationM1R (B-gal)PromoterGal1Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
GTH-ir
Plasmid#81107PurposeGene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FHyTkAX
Plasmid#73901Purposefission yeast drug counter-selectable selection markerDepositorInserthyg-TK
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx2
Plasmid#104471PurposeIntegrating plasmid for overexpression of OM45-FKBPx2.DepositorAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16
Plasmid#45124DepositorInsertDnvLB16
TagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastPromoterGALAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCherry
Plasmid#69857PurposeN-terminal tagging with mCherry using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCherry minus start and stop codon
ExpressionYeastAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBJ1808 pRS416-COF1RFP
Plasmid#37103DepositorInsertCOF1-RFP
TagsRFPExpressionYeastMutationSingle RFP internal insertion at Cof147T48D with …Available SinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only