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pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

Plasmid

#50032

Purpose

Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Publication

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA-HDEL

Expression

Yeast

Promoter

pGAL

Available since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Publication

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pYes2-AtPCS1

Plasmid

#49766

Purpose

Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Publication

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

AtPCS1 (PCS1 Mustard Weed)

Expression

Yeast

Promoter

GAL1

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Publication

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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PRS303-Gal1pr-Mag1-sctetR

Plasmid

#44753

Depositor

Narendra Maheshri

Publication

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

MAG1-sctetR

Expression

Yeast

Promoter

Gal1pr

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Publication

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pXP731

Plasmid

#46057

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pXP811

Plasmid

#46061

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP812

Plasmid

#46062

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP832

Plasmid

#46066

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pR208IIM1

Plasmid

#45352

Depositor

Alexander Varshavsky

Publication

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Expression

Yeast

Mutation

P406S, DE317NK

Promoter

ADH

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pR208IM1

Plasmid

#45351

Depositor

Alexander Varshavsky

Publication

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Expression

Yeast

Mutation

D176E

Promoter

ADH

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoEmerald-Kan

Plasmid

#44946

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEmerald

Expression

Yeast

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-mKO1-SpHis5

Plasmid

#44642

Purpose

Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

Depositor

Kurt Thorn

Insert

mKO1

Expression

Yeast

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-link-mKO1-CaUra3

Plasmid

#44638

Purpose

Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection

Depositor

Kurt Thorn

Insert

mKO1

Expression

Yeast

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Publication

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTH744-CEN-RLuc/slowmaxCFLuc

Plasmid

#38224

Depositor

Tobias von der Haar

Publication

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Publication

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Expression

Bacterial, Mammalian, and Y…

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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