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Addgene

7,941 results

Showing: 6001 - 6050 of 7941 results
  1. pJo55

    Plasmid
    #159623
    Purpose
    An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pIFU002

    Plasmid
    #174299
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Insert
    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, N501Y
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Promoter
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pJL29

    Plasmid
    #174063
    Purpose
    Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.
    Depositor
    Insert
    2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pCyc1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pDB4925

    Plasmid
    #174016
    Purpose
    pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter
    Depositor
    Insert
    Atg38(161-190)
    Use
    Tags
    Pil1, mCherry, Atg38(161-190)
    Expression
    Yeast
    Mutation
    Promoter
    41nmt1
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pGBK CUP1 Nxf1B

    Plasmid
    #170266
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1B
    Depositor
    Insert
    nuclear export factor 1, B6 allele
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pGBK CUP1 Nxf1C

    Plasmid
    #170267
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing full length Nxf1C
    Depositor
    Insert
    nuclear export factor 1, CAST allele
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pGBK CUP1 mCherry

    Plasmid
    #170264
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mCherry
    Depositor
    Insert
    momeric Cherry
    Use
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    CUP1
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pITI2

    Plasmid
    #166095
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Insert
    Cas9-D10A
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. ymTq2-ymNeongreen pDRF1-GW

    Plasmid
    #168069
    Purpose
    FRET fusion between ymTq2 and ymNeongreen for use in yeast
    Depositor
    Insert
    ymTq2-ymNeongreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. ymTq2-ystagRFP pDRF1-GW

    Plasmid
    #168070
    Purpose
    FRET fusion between ymTq2 and ystagRFP for use in yeast
    Depositor
    Insert
    ymTq2-ystagRFPt
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. ystagRFP-T2A-mTq2 pdrf1-GW

    Plasmid
    #168072
    Purpose
    Produces equimolar expression of ystagRFP and mCherry for use in yeast
    Depositor
    Insert
    ystagRFP-T2A-mTq2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. mAvicFP1-T2A-mCherry pdrf1-GW

    Plasmid
    #168073
    Purpose
    Produces equimolar expression of mAvicFP1 and mCherry for use in yeast
    Depositor
    Insert
    mAvicFP1-T2A-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 6001 - 6050 of 7941 results