7,935 results

1. pHES795

   Plasmid

   #87943

   Purpose

   Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   Zif268 DBD - hER LBD - MSN2 AD (ESR1 Budding Yeast, Synthetic, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 (crippled)

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

   Plasmid

   #205256

   Purpose

   S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEZY45

   Plasmid

   #18704

   Purpose

   pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Two-hybrid prey vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM136

   Plasmid

   #133660

   Purpose

   Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p416-GPD-HA-PPK

   Plasmid

   #183943

   Purpose

   Expresses HA-tagged E. coli PPK from yeast GPD promoter

   Depositor

   Michael Downey

   Insert

   PPK (ppk E. coli)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   WT

   Promoter

   GPD

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG416GAL10-HRV-B14_3C

   Plasmid

   #203466

   Purpose

   Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

   Depositor

   Alejandro Chavez

   Insert

   HRV-B14 3C protease (HRVBgp1 )
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHLM

   Plasmid

   #64168

   Purpose

   harbours marker genes HIS3, LEU2 and MET17

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES2.1-TRP1

    Plasmid

    #11007

    Depositor

    Francisco Malagon

    Insert

    TRP1 (TRP1 Budding Yeast)
    

    Use

    Tags

    V5 and his6

    Expression

    Bacterial and Yeast

    Mutation

    ORF-TRP1 without stop codon

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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