8,060 results

1. pZMU-Dest

   Plasmid

   #105089

   Purpose

   Yeast expression vector for Gateway cloning and SUS bridge assays

   Depositor

   Christopher Grefen

   Article

   Grefen et al Methods Mol Biol. 2014;1062:659-78. doi:

   Type

   Empty backbone

   Use

   Tags

   myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB6682

   Plasmid

   #106152

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KBB285

   Plasmid

   #185076

   Purpose

   RFA3-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA3
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. KBB284

   Plasmid

   #185075

   Purpose

   RFA2-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA2
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUB23-R-beta-gal

   Plasmid

   #8818

   Depositor

   Alexander Varshavsky

   Article

   Bachmair et al Science 1986 Oct 10;234(4773):179-86.

   Insert

   beta-gal
   

   Use

   Tags

   Ubiquitin

   Expression

   Yeast

   Mutation

   R (arginine) at amino terminus of beta-gal

   Promoter

   Available since

   Dec. 28, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pKT0146

   Plasmid

   #8742

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   tdimer2
   

   Use

   Tags

   Codon optimized linker

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

   Plasmid

   #124278

   Purpose

   Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

   Depositor

   Arthur Laganowsky

   Article

   Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

   Insert

   GIRK2 (Kcnj6 Mouse)
   

   Use

   Tags

   eGFP-StreptagII-6xHis

   Expression

   Yeast

   Mutation

   R201A

   Promoter

   AOX1

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB4796

   Plasmid

   #106131

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG416GAL-ccdB-EYFP

   Plasmid

   #14219

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMJ273: pRS426-His-MBP-GtFz1_PSP1

    Plasmid

    #205258

    Purpose

    S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ127: pRS426-His-MBP-NlovFz2_PSP1

    Plasmid

    #205257

    Purpose

    S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFP1F5

    Plasmid

    #165064

    Purpose

    Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG413GAL-EGFP-ccdB

    Plasmid

    #14309

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYIAL31

    Plasmid

    #60807

    Purpose

    used to make mutations in yeast POL1

    Depositor

    Youri Pavlov, Thomas Kunkel

    Article

    Pavlov et al Genetics. 2001 Sep;159(1):47-64.

    Insert

    Pol1 (POL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal part only

    Promoter

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUB23-P-beta-gal

    Plasmid

    #8817

    Depositor

    Alexander Varshavsky

    Article

    Bachmair et al Science 1986 Oct 10;234(4773):179-86.

    Insert

    beta-gal
    

    Use

    Tags

    Ubiquitin

    Expression

    Yeast

    Mutation

    P (proline) at amino terminus of beta-gal

    Promoter

    Available since

    Dec. 28, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pTAL6-BB

    Plasmid

    #36034

    Depositor

    Tom Ellis

    Article

    Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Ma

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF1

    Available since

    July 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFTK051

    Plasmid

    #171323

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eBFP fluorescent reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-link-yoPSmOrange-SpHis5

    Plasmid

    #44856

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoPSmOrange
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pI2-MTU-DO-HIS

    Plasmid

    #160016

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB3036(XI-1 MarkerFree)

    Plasmid

    #73274

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge