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pKB12 (pDEST-DHFR F[3] C-term, HygR)

Plasmid

#210486

Purpose

pDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Expression

Yeast

Promoter

ADH1

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW (n1)StayGold-T2A-mCherry

Plasmid

#205495

Purpose

Expression of (n1)StayGold-T2A-mCherry in yeast

Depositor

Bas Teusink

Publication

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

(n1)StayGold-T2A-mCherry

Expression

Yeast

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EMCV_3C

Plasmid

#201941

Purpose

Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EMCV 3C protease (EMCVgp1 )

Expression

Yeast

Promoter

GAL1

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-DENV_NS3-GS-NS2B

Plasmid

#201940

Purpose

Expresses DENV NS2B-NS3 protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DENV NS3-GS-NS2B (POLY )

Expression

Yeast

Promoter

GAL1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-HIV-1_PR

Plasmid

#203493

Purpose

Expresses HIV-1 protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HIV-1 protease

Expression

Yeast

Promoter

GALL

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

Plasmid

#203657

Purpose

Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

GFPMUT3

Expression

Yeast

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Plasmid

#204288

Purpose

yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

Depositor

Linda Huang

Publication

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

Tags

mRuby3

Expression

Yeast

Promoter

pHis3

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook3

Plasmid

#198525

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook3 (HOOK3 Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGBT9-ubiquitin

Plasmid

#196999

Purpose

Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

ubiquitin (UBC Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Promoter

ADH1

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDSM-bc-Ptdh3-CAD-Ttdh1

Plasmid

#127727

Purpose

Yeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.

Depositor

Christopher Voigt

Publication

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

cis-aconitate decarboxylase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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UG75-PAH1-3HA

Plasmid

#184761

Purpose

PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PAH1 (PAH1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

PAH1

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pICE HIS3

Plasmid

#122877

Purpose

HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Km ymScarletI

Plasmid

#193960

Purpose

TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

Depositor

Bas Teusink

Insert

ymScarletI

Expression

Yeast

Promoter

TEF1

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-GFP osTIR1

Plasmid

#182514

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

GFP

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Publication

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Expression

Yeast

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGAL-PDE1

Plasmid

#160388

Purpose

Overxpression of PDE1 in yeast

Depositor

John McCusker

Insert

PDE1

Expression

Yeast

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-PTDH3-SUMO10-6SIM

Plasmid

#190248

Purpose

Expression SUMO10-SIM6 in yeast cells

Depositor

Liam Holt

Publication

Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

Insert

10SUMO-6SIM

Expression

Yeast

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free ClLIS1 (M)

Plasmid

#182487

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Publication

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS(M)-LS

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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