7,935 results

1. pETconNK-TEM1(S70A,D179G)

   Plasmid

   #81166

   Purpose

   pETconNK with TEM1(S70A,D179G) beta-lactamase

   Depositor

   Timothy Whitehead

   Article

   Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

   Insert

   TEM-1 beta-lactamase
   

   Use

   Tags

   c-myc

   Expression

   Bacterial and Yeast

   Mutation

   Deleted the sec transport tag. Plasmid encodes H2…

   Promoter

   GAL1 and gal1

   Available since

   Feb. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAG423GPD-ccdB-EGFP

   Plasmid

   #14198

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cip-pAct1-Mnase-SAT1

   Plasmid

   #162557

   Purpose

   For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

   Depositor

   Adnane Sellam

   Article

   Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

   Insert

   3FLAG-Mnase-SV40
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Actine

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LPH2781 - Ub-K63R

   Plasmid

   #32160

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K63R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCfB3045(gRNA XI-3)

   Plasmid

   #73287

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XI-3

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pESC/mCitrine

   Plasmid

   #117996

   Purpose

   Expression of mCitrine controlled by the Gal1 promoter

   Depositor

   Mathias Pribil

   Article

   Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   mCitrine
   

   Use

   Yeast/bacteria binary vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNH605-CCAAT-YFP

   Plasmid

   #172132

   Purpose

   Fluorescent reporter for HAP complex activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xCCAAT-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PhyB-mCherry-CAAX

   Plasmid

   #51567

   Purpose

   Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   CAAX and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046233

   Plasmid

   #149032

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFA6a-6xGLY-T7-hphMX4

    Plasmid

    #20761

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge