8,044 results
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Plasmid#184966PurposeExpress -Eco1 dead RT and ncRNA in yeasstDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
UseTagsExpressionYeastMutationHuman codon optimized RT, YXDD catalytic region m…PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.005
Plasmid#184971PurposeExpress Cas9 in yeastDepositorInsertIntegrating inducible cassette: Cas9
UseTagsSV40NLSExpressionYeastMutationPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.71
Plasmid#184983PurposeExpress -Eco1 RT/Cas9 fusion, using linker1DepositorInsertEco1RT-fusion_linker1-Cas9
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.112
Plasmid#184981PurposeExpress -Eco1 FAA1 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
UseTagsExpressionYeastMutationFAA1 donor P233stopPromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.113
Plasmid#184982PurposeTest effect of extending a1/a2 on FAA1 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationFAA1 donor P233stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.002
Plasmid#184972PurposeExpress -Eco1 ADE2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
UseTagsExpressionYeastMutationADE2 donor P272stopPromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.110
Plasmid#184979PurposeExpress -Eco1 TRP2 editing ncRNA and gRNADepositorInsertEco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
UseTagsExpressionYeastMutationTRP2 donor E64stopPromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.039
Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
UseTagsExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yeast_Target-AID2S
Plasmid#188647PurposeExpressing Target-AID2S in yeastsDepositorInsertnCas9-AID2S-UGI
UseTagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby2
Plasmid#176550PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mWasabi
Plasmid#176551PurposeExpression of mWasabi for auxotrophic selection in the absence of histidineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-mWasabi
Plasmid#176553PurposeExpression of mWasabi for auxotrophic selection in the absence of uracilDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mWasabi
Plasmid#176554PurposeExpression of mWasabi for auxotrophic selection in the absence of methionineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-TagRFP657
Plasmid#176562PurposeExpression of TagRFP657 for auxotrophic selection in the absence of uracilDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-YLL058W-lt
Plasmid#176564PurposeExpression of YLL058W for Lanchea thermotolerans for auxotrophic selection in the absence of uracilDepositorInsertYLL058W-lt
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-YLL058W-zr
Plasmid#176565PurposeExpression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracilDepositorInsertYLL058W-zr
UseTagsExpressionYeastMutationCodon optimised for expression in S.cerevisaePromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-Sapphire
Plasmid#176566PurposeExpression of Sapphire for auxotrophic selection in the absence of lysineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-Sapphire
Plasmid#176567PurposeExpression of Sapphire for auxotrophic selection in the absence of uracilDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-Sapphire
Plasmid#176569PurposeExpression of Sapphire for auxotrophic selection in the absence of methionineDepositorInsertyETSapphire
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
LDB73
Plasmid#185437PurposeNUP1 CEN URA3 vector for synthetic lethal sectoring assays and screeningDepositorInsertNUP1
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIFEX-A2aGH
Plasmid#160542PurposepITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX1-A2aGH
Plasmid#160543PurposepYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX2-A2aGH
Plasmid#160544PurposepYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFEX3-A2aGH
Plasmid#160545PurposepYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX1-A2aGH
Plasmid#160546PurposepYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX2-A2aGH
Plasmid#160547PurposepYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFEX3-A2aGH
Plasmid#160548PurposepYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.DepositorInsertUseTagsExpressionYeastMutationN/APromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
UseTagsmScarlet fused to FKBPExpressionYeastMutationPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1-FLAG
Plasmid#189725PurposeEnables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterHTA1Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- TEF2prom-TIR1-FLAG
Plasmid#189726PurposeEnables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterTEF2Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Kan
Plasmid#188928PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Kan
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-GS
Plasmid#188939PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-GS
Plasmid#188938PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-GS
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-DHFR-3xHA
Plasmid#188934PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-DHFR-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-URA
Plasmid#188927PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-URA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-NAT
Plasmid#188926PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-NAT
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Phleomycin
Plasmid#188925PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Phleomycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-eDHFR-Hygromycin
Plasmid#188924PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-eDHFR-Hygromycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Phleomycin
Plasmid#188920PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Phleomycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-Hygromycin
Plasmid#188919PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-Hygromycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only