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pDRF1-GW mVenus-T2A-mTurquoise2

Plasmid

#118434

Purpose

Produces equimolar expression of mVenus and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mVenus-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tdTomato-T2A-mTurquoise2

Plasmid

#118439

Purpose

Produces equimolar expression of tdTomato and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tdTomato-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

nmt41

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Publication

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1673

Plasmid

#112047

Purpose

CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SCT1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Publication

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-Erg11-iGFPx3

Plasmid

#115422

Purpose

Express Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Erg11-iGFPx3

Tags

iGFP

Expression

Yeast

Promoter

Endogenous

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac204-pOst1-ESCargo

Plasmid

#115420

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Publication

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Publication

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Expression

Bacterial and Yeast

Promoter

ATG41 endogenous promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDR217

Plasmid

#113872

Purpose

expression of Cas9 programming sgRNA9 and sgRNA10 targetting MPH2-3 and MAL11 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Publication

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA9-MPH2-3 sgRNA10-MAL11

Use

CRISPR

Expression

Yeast

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Publication

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Expression

Yeast

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Publication

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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yCp50-PRP2

Plasmid

#111304

Purpose

Prp2 in yCp50 plasmid

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP2

Expression

Yeast

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSE360-Prp22

Plasmid

#111284

Purpose

Prp22 WT gene in pSE360 backbone

Depositor

Christine Guthrie

Publication

Noble et al Genetics. 1996 May;143(1):67-80.

Insert

PRP22

Expression

Yeast

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHM4 (U6 in pSE360)

Plasmid

#111281

Purpose

U6 snRNA coding sequence in pSE360(Ura) plasmid

Depositor

Christine Guthrie

Publication

Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

Insert

SNR6

Expression

Yeast

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pJOG418

Plasmid

#105342

Purpose

Yeast two hybrid

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Type

Empty backbone

Expression

Yeast

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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