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pMJA104

Plasmid

#128520

Purpose

Expresses amyloid human Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Publication

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme I56T (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA065

Plasmid

#128517

Purpose

Expresses Ber e 1 in Pichia

Depositor

Marcos Alcocer

Publication

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

Ber e 1 a allergenic 2S albumin from Brazil nut

Expression

Yeast

Promoter

AOX1

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB4929

Plasmid

#126751

Purpose

Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

ALD6

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Publication

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWS033

Plasmid

#123056

Purpose

Green fluorescent protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

sfGFP [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRC063

Plasmid

#123057

Purpose

Blue fluorescent protein

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

mTagBFP2 [3]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS926

Plasmid

#123052

Purpose

LexA bacterial repressor

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexA-NLS [3a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Expression

Yeast

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA (405)

Plasmid

#121417

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Publication

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1promoter-Dhh1 ORF-PA-ADH1 terminator (DHH1 Budding Yeast)

Expression

Yeast

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17

Plasmid

#121394

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Publication

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mVenus-T2A-mTurquoise2

Plasmid

#118434

Purpose

Produces equimolar expression of mVenus and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mVenus-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW tdTomato-T2A-mTurquoise2

Plasmid

#118439

Purpose

Produces equimolar expression of tdTomato and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

tdTomato-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

nmt41

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Publication

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSec7-Halox2

Plasmid

#115425

Purpose

Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-Halox2

Tags

HaloTag

Expression

Yeast

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-vps10-104

Plasmid

#115426

Purpose

Generate the vps10-104 allele via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Publication

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

vps10-104

Expression

Yeast

Mutation

domain 1 deletion

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJW1673

Plasmid

#112047

Purpose

CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SCT1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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