8,133 results

1. YIplac211-GGA2-HaloTagx2

   Plasmid

   #140150

   Purpose

   Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   GGA2
   

   Expression

   Yeast

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-FTKanAX

   Plasmid

   #73900

   Purpose

   fission yeast drug counter-selectable selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   TK-kan
   

   Expression

   Yeast

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HsCERK

   Plasmid

   #118600

   Purpose

   Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens ceramide kinase
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1, 10

   Available Since

   Feb. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG306GAL-ccdB-EYFP

   Plasmid

   #14211

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAD-GH-MEFV

   Plasmid

   #92106

   Purpose

   Yeast two hybrid

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   MEFV (MEFV Human)
   

   Expression

   Yeast

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-mKO1-SpHis5

   Plasmid

   #44642

   Purpose

   Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection

   Depositor

   Kurt Thorn

   Insert

   mKO1
   

   Expression

   Yeast

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. FRP1625

   Plasmid

   #64537

   Purpose

   Expresses LexA-ER-B42 in yeast cells

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Insert

   LexA-ER-B42
   

   Expression

   Yeast

   Promoter

   ACT1

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG426GPD-ccdB-TAP

   Plasmid

   #14276

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   TAP

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK370

   Plasmid

   #73595

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitope

   Depositor

   T. J. Kappock

   Inserts

   delta-12 fatty acid acetylenase
   delta-12 fatty acid desaturase (form 2)
   

   Tags

   FLAG tag and c-myc tag

   Expression

   Yeast

   Mutation

   Silent mutation in Arg299 codon.

   Promoter

   GAL1 and GAL10

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS405 VAC8-RFP

    Plasmid

    #166841

    Purpose

    Expresses Vac8-RFP in yeasts

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Tags

    RFP

    Expression

    Yeast

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-GBP-RFP-hphMX6

    Plasmid

    #105161

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

    Insert

    GBP-RFP-hphMX6
    

    Tags

    GBP-RFP

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAH2

    Plasmid

    #41826

    Purpose

    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

    Depositor

    Melissa Moore

    Article

    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

    Type

    Empty backbone

    Use

    Pcr template

    Expression

    Bacterial and Yeast

    Promoter

    T7, TEF

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDE722

    Plasmid

    #103022

    Purpose

    expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHCAN1-4.S (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. D588 lys2::HIS3 Disruptor Converter

    Plasmid

    #51675

    Purpose

    Yeast marker swap plasmid for converting LYS2 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML104-kanR1136

    Plasmid

    #162040

    Purpose

    Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    kanR1136 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW8-aid-V5-Turg1:kanMX6

    Plasmid

    #169357

    Purpose

    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe

    Depositor

    Tony Carr

    Insert

    auxin-inducible degron (aid)
    

    Tags

    1XV5

    Expression

    Yeast

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKT0174

    Plasmid

    #8720

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. PP363

    Plasmid

    #78984

    Purpose

    255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Inserts

    rHGH
    IFNa2b
    

    Expression

    Yeast

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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