7,935 results

1. pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

   Plasmid

   #178985

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0782

   Plasmid

   #133514

   Purpose

   Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ-pre-pro-alphaf-E2-Crimson

   Plasmid

   #117658

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHflu2

   Plasmid

   #203880

   Purpose

   pHI1 with insertion of ampicillin-resistance gene

   Depositor

   Bogumil Karas

   Insert

   broad-spectrum class A beta-lactamase TEM-116
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Yeast_Target-AID3S

   Plasmid

   #188646

   Purpose

   Expresses Target-AID3S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MET-HYGRO

   Plasmid

   #218113

   Purpose

   Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. LYS-Leu2

   Plasmid

   #218114

   Purpose

   Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY3

    Plasmid

    #218116

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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