8,057 results

1. pSM004

   Plasmid

   #122937

   Purpose

   Expression of the marked mRNA under the control of substitutional promoters.

   Depositor

   Attila Becskei

   Article

   Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

   Insert

   YML065W (Marked)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

   Promoter

   [tetO2]4inPgal1

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSV_228

   Plasmid

   #122933

   Purpose

   Expression of the marked mRNA under the control of substitutional promoters.

   Depositor

   Attila Becskei

   Article

   Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

   Insert

   YBR025C (Marked)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

   Promoter

   [tetO2]4inPgal1

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSV_229

   Plasmid

   #122934

   Purpose

   Expression of the marked mRNA under the control of substitutional promoters.

   Depositor

   Attila Becskei

   Article

   Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

   Insert

   YBR026C (Marked)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

   Promoter

   [tetO2]4inPgal1

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS-ATG1(mut)(406)

   Plasmid

   #122069

   Purpose

   Expressing a mutant ATG1

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon changes without changing the amino acid seq…

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYZ292

   Plasmid

   #102686

   Purpose

   S. pombe gRNA entry vector with Cas9 - ScLEU2 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWS1859

   Plasmid

   #123065

   Purpose

   Positive regulator of Ste20

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Ste50 [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWS511

   Plasmid

   #123063

   Purpose

   Negative regulator of Ste12

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Dig2 [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWS510

   Plasmid

   #123062

   Purpose

   Negative regulator of Ste12

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Dig1 [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWS509

   Plasmid

   #123061

   Purpose

   Negative regulator of Fus3

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Msg5 [3]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWS081

    Plasmid

    #123060

    Purpose

    Regulator of G protein signalling

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Sst2 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pWS930

    Plasmid

    #123054

    Purpose

    Beta-estradiol inducible DNA binding domain

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Z3E [3a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWS626

    Plasmid

    #123045

    Purpose

    P-factor GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    Mam2 [3]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWS495

    Plasmid

    #123043

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pRNR2m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWS497

    Plasmid

    #123042

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pCUP1m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWS496

    Plasmid

    #123041

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pCYC1m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pWS498

    Plasmid

    #123040

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pPHO5m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS486

    Plasmid

    #123039

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pGAL1m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWS493

    Plasmid

    #123037

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pALD6m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS487

    Plasmid

    #123036

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pTDH3m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWS491

    Plasmid

    #123035

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pTEF2m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pWS494

    Plasmid

    #123034

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pRNR1m [2b]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pWS485

    Plasmid

    #123033

    Purpose

    Core promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (1x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWS480

    Plasmid

    #123028

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (8x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pWS1078

    Plasmid

    #123027

    Purpose

    Z3E promoter

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pZ3 [2]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDRF1-GW YPET-T2A-mTurquoise2

    Plasmid

    #118450

    Purpose

    Produces equimolar expression of YPET and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    YPET-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDRF1-GW yeVenus-T2A-mTurquoise2

    Plasmid

    #118440

    Purpose

    Produces equimolar expression of yeVenus and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yeVenus-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDRF1-GW tagRFP-T2A-mTurquoise2

    Plasmid

    #118437

    Purpose

    Produces equimolar expression of tagRFP and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    tagRFP-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDRF1-GW mTurquoise2-T2A-mcherry

    Plasmid

    #118433

    Purpose

    Produces equimolar expression of mTq2 and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    mTurquoise2-T2A-mcherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDRF1-GW Clover-T2A-mTurquoise2

    Plasmid

    #118425

    Purpose

    Produces equimolar expression of Clover and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    Clover-T2A-mTq2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGFP-ATG13[3SD](315)

    Plasmid

    #122074

    Purpose

    Expressing GFP-Atg13[3SD]

    Depositor

    Daniel Klionsky

    Article

    Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

    Insert

    ATG13
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Serine 646 to aspartic acid; Serine 649 to aspart…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGFP-ATG13[3SA](315)

    Plasmid

    #122073

    Purpose

    Expressing GFP-Atg13[3SA]

    Depositor

    Daniel Klionsky

    Article

    Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

    Insert

    ATG13
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Serine 646 to alanine; Serine 649 to alanine; Ser…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCuHA-SpAtg17(416)

    Plasmid

    #122079

    Purpose

    Expressing S. pombe HA-Atg17

    Depositor

    Daniel Klionsky

    Article

    Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

    Insert

    SpATG17 (atg17 Fission Yeast)
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCuHA-SpAtg101(414)

    Plasmid

    #122081

    Purpose

    Expressing S. pombe HA-Atg101

    Depositor

    Daniel Klionsky

    Article

    Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

    Insert

    SpATG101
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS-ATG1-ATG7 3'UTR(406)

    Plasmid

    #122070

    Purpose

    Expressing ATG1 with ATG7 3'UTR

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    serine/threonine protein kinase ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The native 3'UTR was replaced by ATG7 3'…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCu-SpAtg17(416)

    Plasmid

    #122078

    Purpose

    Expressing S. pombe Atg17

    Depositor

    Daniel Klionsky

    Article

    Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

    Insert

    SpATG17 (atg17 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS-ATG7p-ATG1(406)

    Plasmid

    #122071

    Purpose

    Expressing ATG1

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The native promoter was replaced by ATG7 promoter…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRS-Dhh1-PA(D195A,E196A)(405)

    Plasmid

    #122065

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Aspartic acid 195 to alanine; Glutamic acid 196 t…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS-Dhh1-PA(S226A,T228A)(405)

    Plasmid

    #122066

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Serine 226 to alanine; Threonine 228 to alanine

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGBDU-Atg17(F317D, I325A)

    Plasmid

    #122275

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Aspartic acid; Isoleucine 32…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGAD-Atg17(F317H)

    Plasmid

    #122269

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Phenylalanine 317 to Histidine

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge