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Plasmid#133500PurposeExpression of sfGFP targeted to the nucleus with one NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-sfGFP-NLSSV40-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pESC-LEU (V5 and HA)
Plasmid#222486PurposeCo-expression of two genes in yeast with V5 and HA tags and LEU2 selectionDepositorTypeEmpty backboneUseTagsHA and V5ExpressionYeastMutationPromoterAvailable sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV1090
Plasmid#111427PurposegRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)DepositorInsertsgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNK5867
Plasmid#219751PurposepGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expressionDepositorInsertpGAP - mcitHispS - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNK1508
Plasmid#219750PurposepGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expressionDepositorInsertpGAP - npgA - tAOX
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG205MX
Plasmid#64535PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG206MX
Plasmid#64536PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-YFP-yeastSMASh
Plasmid#68855PurposeExpresses SMASh tag fused to YFP at C terminus in yeastDepositorInsertYFP-yeastSMASh
UseTagsSMASh tagExpressionYeastMutationPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZalphaB-SapL3
Plasmid#78171PurposeExpression of Saporin L3 in pichiaDepositorInsertSaporin L3
UseTagsalpha factorExpressionYeastMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRG201
Plasmid#64394PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCAS9c
Plasmid#141250PurposeConstitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1 (Cas9), pSNR52 (gRNA)Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-G76V-GFP
Plasmid#11954DepositorInsertUb-G76V-GFP
UseTagsExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGAL-GFP kan
Plasmid#122060Purposegalactose inducible expression of GFP in yeast. marked with URA3DepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterGAL1Available sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mNeonGreen-KanMX6
Plasmid#129099PurposeFor tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-eGFP
Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTagsExpressionYeastMutationPromoterGAPAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only