8,057 results

1. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pWS082

   Plasmid

   #90516

   Purpose

   sgRNA entry vector

   Depositor

   Tom Ellis

   Insert

   Yeast sgRNA expression vector
   

   Use

   CRISPR; Yeast gap repair

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-5FLAG-natMX6

   Plasmid

   #19343

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB4586

   Plasmid

   #106124

   Purpose

   EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDR195

   Plasmid

   #36028

   Depositor

   Dominique Loque, Wolf Frommer

   Article

   Rentsch et al FEBS Lett. 1995 Aug 21;370(3):264-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPMA1

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHYX137

   Plasmid

   #202814

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScPCK1

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHYX138

   Plasmid

   #202815

   Purpose

   Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter ADH2. URA3 selection.

   Depositor

   Jean-Félix Dallery

   Article

   Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScADH2

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. VanR-wt

   Plasmid

   #153339

   Purpose

   Wild type VanR from Caulobacter crescentus. The gene is under the control of the TEF1 promoter

   Depositor

   Michael Jensen

   Article

   et al Biotechnology Notes, Volume 1, 2020, Pag

   Insert

   VanR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS426GFP-2×PH(PLCδ)

   Plasmid

   #36092

   Depositor

   Scott Emr

   Article

   Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

   Insert

   2xPH(PLCdelta) fusion
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   contains aa11-140 of PLCδ (including the PH domai…

   Promoter

   PRC1

   Available since

   June 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p413 TEF NAPstar4.3

    Plasmid

    #232743

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar4.3 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar4.3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-mNeonGreen-KanMX6

    Plasmid

    #129099

    Purpose

    For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

    Depositor

    Wei-Lih Lee

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Use

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVS1

    Plasmid

    #33143

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Insert

    LacO (256 copies)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. YIp204-PADH1-atTIR1-9myc

    Plasmid

    #99532

    Purpose

    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    9xMyc

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GAL-Aur1-2GFP-Hygromycin

    Plasmid

    #184775

    Purpose

    Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proGAL-Aur1 (AUR1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXN21-Dest

    Plasmid

    #105086

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lifeact-3xmNG::TRP1

    Plasmid

    #227593

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::TRP1
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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