8,060 results

1. pAPInt-mScarlet-G418

   Plasmid

   #236493

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAPInt-mScarlet-Hyg

   Plasmid

   #236492

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAPInt-GFP-Hyg

   Plasmid

   #236477

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAPInt-sfGFP-Nat

   Plasmid

   #236479

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAPInt-mNeonGreen-Nat

   Plasmid

   #236482

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAPInt-GFP-G418

   Plasmid

   #236478

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ACT1-CUP1 9bp dist

   Plasmid

   #234131

   Purpose

   PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

   Depositor

   Aaron Hoskins

   Insert

   ACT1-CUP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Intron truncation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ACT1-CUP1 12bp dist

   Plasmid

   #234130

   Purpose

   PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

   Depositor

   Aaron Hoskins

   Insert

   ACT1-CUP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Intron truncation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ACT1-CUP1 27bp dist

   Plasmid

   #234129

   Purpose

   PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

   Depositor

   Aaron Hoskins

   Insert

   ACT1-CUP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Intron truncation

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ACT1-CUP1 15bp dist

    Plasmid

    #234128

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ACT1-CUP1 42bp dist

    Plasmid

    #234127

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ACT1-CUP1 50bp dist

    Plasmid

    #234112

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ACT1-CUP1 46bp dist

    Plasmid

    #234111

    Purpose

    PCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backbone

    Depositor

    Aaron Hoskins

    Insert

    ACT1-CUP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Intron truncation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6-CFAPoptim-NAT

    Plasmid

    #221129

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6-CFAPoptim-KAN

    Plasmid

    #221128

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p413 TEF NAPstar1

    Plasmid

    #232738

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p413 TEF NAPstar2

    Plasmid

    #232739

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p413 TEF NAPstar4

    Plasmid

    #232741

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p413 TEF NAPstar6

    Plasmid

    #232742

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p413 TEF NAPstar7

    Plasmid

    #232744

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.

    Depositor

    Bruce Morgan

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    NAPstar7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge