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Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only
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pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHM2404
Plasmid#173441PurposepRS316-mNeonGreen-CBP-2XFLAG-NAT1, contains C. neoformans optimized mNeonGreen and NAT1 marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-NAT1
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-3xFLAG-hphMX4
Plasmid#20755DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
UseTagsCBP-2xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHLUM (version 2)
Plasmid#64166Purposeharbours marker genes HIS3, LEU2, URA3 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only