7,943 results

1. pCfB3042(gRNA X-4)

   Plasmid

   #73284

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   guiding RNA
   

   Use

   CRISPR; Grna

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS7

   Plasmid

   #83774

   Purpose

   Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

   Depositor

   Eric Muller

   Article

   Hailey et al Methods Enzymol. 2002;351:34-49.

   Insert

   Venus
   

   Use

   Tags

   Venus

   Expression

   Yeast

   Mutation

   F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

   Promoter

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-yomWasabi-SpHis5

   Plasmid

   #44850

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomWasabi
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416 Gal-RNQ1-YFP

   Plasmid

   #18685

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 (RNQ1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

5. p406GALL

   Plasmid

   #17434

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBW-m4046464

   Plasmid

   #148970

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pYTK096

   Plasmid

   #65203

   Purpose

   Pre-Assembled Ura3 integration vector to be used in the Dueber YTK system.

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFTK049

   Plasmid

   #171321

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   mCherry fluorescent reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available since

   Dec. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHM4 (U6 in pSE360)

   Plasmid

   #111281

   Purpose

   U6 snRNA coding sequence in pSE360(Ura) plasmid

   Depositor

   Christine Guthrie

   Article

   Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

   Insert

   SNR6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS316 GAL cluster

    Plasmid

    #61925

    Purpose

    Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316

    Depositor

    Jon Houseley

    Article

    Cruz et al Elife. 2014 Feb 11;3:e01581. doi: 10.755

    Inserts

    GAL1 (GAL1 Budding Yeast)
    GAL10 (GAL10 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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