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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJZC593

Plasmid

#62319

Purpose

sgRNA with MS2-PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + MS2-PP7 RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAS2

Plasmid

#12389

Depositor

Nick Rhind

Article

Sigova et al Genes Dev. 2004 Oct 1. 18(19):2359-67.

Insert

green fluorescent protein

Expression

Yeast

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Expression

Yeast

Promoter

yeast PKC

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pNC824

Plasmid

#20384

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

UAS cassette (FUS1)

Expression

Yeast

Mutation

X=UbiY (see schematic)

Available Since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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GAL 103Q+ProCFPp303

Plasmid

#15575

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Tags

CFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pUB23-L-beta-gal

Plasmid

#8820

Depositor

Alexander Varshavsky

Article

Bachmair et al Science 1986 Oct 10;234(4773):179-86.

Insert

beta-gal

Tags

Ubiquitin

Expression

Yeast

Mutation

L (leucine) at amino terminus of beta-gal

Available Since

Dec. 28, 2005

Availability

Academic Institutions and Nonprofits only
pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-CPR

Plasmid

#126742

Purpose

yeast plasmid overexpressing CPR

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

cpr

Tags

6xHis

Expression

Yeast

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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