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Plasmid#213783PurposeIntegrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutationsDepositorInsertura3*
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
JME4580
Plasmid#129659PurposeHPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
JME4472
Plasmid#129658PurposeURA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGateDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
POM43-mTFP1
Plasmid#69853PurposeN-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmTFP1 minus start and stop codon
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYD1
Plasmid#73447Purposeexpression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cellsDepositorTypeEmpty backboneUseTags6xHis, AGA2, T7 peptide, V5 tag, and Xpress tagExpressionYeastMutationPromoterGAL1Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
UseTagsExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTurbo-V5_pRS415
Plasmid#107168Purposeexpresses V5-tagged miniTurbo in the yeast cytosolDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionYeastMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p406ADH1
Plasmid#15974DepositorInsertADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationADH1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
SJZ9-amdS
Plasmid#228261PurposeTemplate plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen sourceDepositorInsertamdS
UseTagsExpressionYeastMutationPromoterENO2 S.c.Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
p404TEF1
Plasmid#15972DepositorInsertTEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTEF1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-URA3
Plasmid#224075PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)
Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB769
Plasmid#60138PurposeRed click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBR
UseTagsExpressionYeastMutationPromoterMET17prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNX32-Dest
Plasmid#105083PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXN22-Dest
Plasmid#105085PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneUseTagsNubG-3xHAExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorInsertsUseTagsExpressionYeastMutationPromoterADH1 and GPDAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only