8,057 results

1. pCfB8748

   Plasmid

   #126918

   Purpose

   Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   XdCrtE
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG706_lexO-SbfI_LexA-TF_LEU2MX

   Plasmid

   #154818

   Purpose

   Inducible expression of SbfI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   SbfI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCTCON2-BYG

   Plasmid

   #158126

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   mTagBFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

   Plasmid

   #86424

   Purpose

   PCR tagging module with yeast codon optimized mTurquoise2

   Depositor

   Sue Jaspersen

   Article

   Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG426GPD-ccdB-DsRed

   Plasmid

   #14372

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

   Plasmid

   #24378

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::p150yeGFP-DHFR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-X0

   Plasmid

   #115565

   Purpose

   Control vector expressing GFP alone without any consensus repeats

   Depositor

   Michael Downey

   Article

   Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS315e_pGal-nCas9(D10A)-PmCDA1

   Plasmid

   #79617

   Purpose

   Expresses nCas9(D10A)-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A for nickase Cas9

   Promoter

   pGal1

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. YCplac22-ENT2

   Plasmid

   #26684

   Depositor

   Linda Hicke

   Article

   Dores et al Traffic. 2010 Jan . 11(1):151-60.

   Insert

   ENT2 (ENT2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pPing

    Plasmid

    #47100

    Purpose

    Expresses the Ping open reading frame 1 (ORF1) and transposase from rice to allow mPing movement. The vector contains ORF1, transposase, mPing element and hph for hygromycin selection.

    Depositor

    Wayne Parrott

    Article

    Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

    Inserts

    Ping cDNA
    mPing
    hygromycin resistance gene
    

    Use

    Plant expression

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    CaMV35s and StUbi3

    Available since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVG10

    Plasmid

    #164702

    Purpose

    yEVenus under the control of GALL promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    GALLpr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUP3

    Plasmid

    #92174

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-yomRuby3-SpHIS5

    Plasmid

    #204287

    Purpose

    For C-terminal tagging using yeast optimized mRuby3

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pFA6-yomRuby3-SpHIS5
    

    Use

    Tags

    yomRuby3

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-yEmCFP-Hygro

    Plasmid

    #44644

    Depositor

    Kurt Thorn

    Insert

    yEmCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A206R

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYX233-b2delta-DHFR

    Plasmid

    #163760

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2delta-DHFR (Dhfr Budding Yeast, Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS405-LEU2-TEF-KpolDcr1v2

    Plasmid

    #85193

    Purpose

    Integrating plasmid, K. polysporus Dcr1 under TEF promoter

    Depositor

    David Bartel

    Article

    Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

    Insert

    Dcr1
    

    Use

    Integrating, shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

    Plasmid

    #173455

    Purpose

    Contains mScarlet-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIYC08

    Plasmid

    #64743

    Purpose

    Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

    Depositor

    Jens Nielsen

    Article

    Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

    Insert

    ADH2, ALD6, acsL641P, ERG10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge