8,436 results
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Plasmid#118459PurposeYeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection. *An updated version of this plasmid is available: #168058 pFA6a-link-ymVenus-URA3.DepositorInsertymVenus
ExpressionYeastAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf-Btl2
Plasmid#117664PurposeStudy secretion efficiency of Btl2DepositorInsertpre-pro-alphaf-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDB24
Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneTags10-His and Smt3ExpressionBacterial and YeastPromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDP013
Plasmid#103873PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPRExpressionYeastPromoterScTDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-3GFP-kanMX6
Plasmid#105148PurposeYeast gene targetingDepositorInsert3GFP-kanMX6
Tags3GFPExpressionBacterial and YeastAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionWorm and YeastAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4279
Plasmid#98698PurposeA control plasmid containing the intact bsdMX markerDepositorInsertCas9
UseCRISPRExpressionYeastAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
Tags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR FLIP43 GPS1
Plasmid#101084PurposeGA biosensor expression plasmidDepositorInsertGPS1
Tags6HIS and cMycExpressionYeastPromoterpPMA1Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS423-CUP1-His-Ubi
Plasmid#99541PurposeYeast episomal vector for expression of His-tagged ubiquitin; HIS3 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YEp195-CUP1-His-Smt3
Plasmid#99538PurposeYeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 markerDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E2-Crimson-FKBP(M)
Plasmid#85002PurposeExpresses E2-Crimson-FKBP(M)DepositorInsertE2-Crimson-FKBP(M)
ExpressionYeastAvailable sinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2904(XI-3 MarkerFree)
Plasmid#73276PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCfB3042(gRNA X-4)
Plasmid#73284PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-2DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only