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pABY-T4

Plasmid

#201767

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-sctetR-FokI

Plasmid

#44756

Depositor

Narendra Maheshri

Article

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

sctetR-FokI (fokI Synthetic)

Expression

Yeast

Promoter

Gal1pr

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pG/N795

Plasmid

#108220

Purpose

Yeast expression vector for rat glucocorticoid receptor

Depositor

Didier Picard, Keith Yamamoto

Article

Schena et al Methods Enzymol. 1991;194:389-98.

Insert

Rat glucocorticoid receptor

Expression

Yeast

Promoter

TDH3

Available Since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-G418

Plasmid

#236481

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMG343

Plasmid

#176183

Purpose

preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2+ccdB dropout cassette

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

Plasmid

#116884

Purpose

FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RLR)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal-RNQ1-YFP

Plasmid

#18686

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
GFP-ATG8(414)/GFP-AUT7(414)

Plasmid

#49424

Purpose

Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

Depositor

Daniel Klionsky

Article

Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

Insert

autophagy-related 8 (ATG8 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Promoter

ATG8

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pTH650-RLuc/staFLuc

Plasmid

#29696

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pMTKM05

Plasmid

#233477

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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p403 – TDH3-MTS-mCherry-GFP1-10-His

Plasmid

#177730

Purpose

mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

mCherry-GFP1-10

Tags

Mitochondrial Targeting Sequence

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

Plasmid

#173454

Purpose

Contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pKS1576

Plasmid

#239524

Purpose

Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur

Depositor

John Dueber

Insert

ScVba2p-mTurquoise2-2xCrk_SH3

Expression

Yeast

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJJB1577

Plasmid

#218619

Purpose

Express Pex5, Pex2, Pex17, and Pex10

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex5

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1578

Plasmid

#218620

Purpose

Express Pex22, Pex6, Pex15, and Pex8

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKT127-SEP

Plasmid

#41899

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Tags

superecliptic GFP (SEP)

Expression

Yeast

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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