8,057 results

1. pFA6a- mYFP-kanMX6

   Plasmid

   #105147

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mYFP-kanMX6
   

   Use

   Tags

   mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG/ER(G)

   Plasmid

   #108219

   Purpose

   Yeast expression vector for human estrogen receptor alpha

   Depositor

   Didier Picard

   Article

   Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

   Insert

   Human estrogen receptor a (ESR1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJO629

   Plasmid

   #104342

   Purpose

   Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

   Insert

   pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoTagRFP-T-SpHis5

   Plasmid

   #44842

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK

   Plasmid

   #64188

   Purpose

   harbours marker gene LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

   Plasmid

   #179079

   Purpose

   Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-SKII-3XHA-HALO-Hygromycin

   Plasmid

   #188929

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Hygromycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS305-mAID-LaM2

   Plasmid

   #198413

   Purpose

   Express mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeast

   Depositor

   Takumi Kamura

   Article

   Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

   Insert

   mAID-Nb (LaM2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS712

    Plasmid

    #213041

    Purpose

    CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

    Depositor

    Rebecca Shapiro

    Article

    Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FRP935

    Plasmid

    #159302

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p413TEF-PKM2

    Plasmid

    #34608

    Depositor

    Markus Ralser

    Article

    Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIM573

    Plasmid

    #74637

    Purpose

    alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C-SWAT AID*-GFP osTIR1

    Plasmid

    #182514

    Purpose

    C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    AID*
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-yoTagRFP657-SpHis5

    Plasmid

    #44846

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pK_P-GAP-ACC1*

    Plasmid

    #126740

    Purpose

    yeast plasmid overexpressing ACC1 from Komagataella phaffii

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    acc1*
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T3452G

    Promoter

    GAP

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. THI VP64 pALD4 T-

    Plasmid

    #161481

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge