8,086 results
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Plasmid#44647DepositorInsertyEmCFP
UseTagsExpressionYeastMutationPromoterAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACT2.2
Plasmid#11343DepositorTypeEmpty backboneUseTagsGal4 Activation DomainExpressionYeastMutationPromoterAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R1
Plasmid#161493PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R1_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R1 in the RIB1 promoter)
UseTagsExpressionYeastMutationPromoterpGAP, pPOR1, and pTEF2Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIW447-CRISPR XYL2
Plasmid#98906PurposeK. marxianus CRISPR Plasmid for XYL2 editingDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastMutationPromoterAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- PGK1prom-TIR1-FLAG
Plasmid#189724PurposeEnables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)DepositorInsertOsTIR1
UseTagsExpressionYeastMutationPromoterPGK1 promoterAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3
Plasmid#203657PurposeYeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsGFPMUT3ExpressionYeastMutationPromoterAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP-T-SpHis5
Plasmid#44842PurposeYeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selectionDepositorInsertyoTagRFP-T
UseTagsExpressionYeastMutationPromoterAvailable SinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3B
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3B
UseTagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationPromoterADH1Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0620
Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable SinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
UseTagsExpressionYeastMutationPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJO629
Plasmid#104342PurposeExpress yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertpTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYM29-BirA*
Plasmid#160291PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1DepositorInsertBirA*
UseTagsExpressionYeastMutationPromoterAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-G418
Plasmid#160023PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natCX
Plasmid#73893Purposefission yeast drug selection markerDepositorInsertnatCX
UseTagsExpressionYeastMutationPromoterAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWS2247
Plasmid#123026PurposeTet promoter (TetO(x7)-pLEU2m)DepositorInsertpTet [2]
UseTagsExpressionYeastMutationPromoterAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only