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ZJOM57

Plasmid

#133656

Purpose

Integration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-PEX3-DuDre (PEX3 Budding Yeast)

Use

Tags

DuDre

Expression

Yeast

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGal4BD-CRY2

Plasmid

#28243

Purpose

Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Gal4BD

Expression

Yeast

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECitrine-Hygro

Plasmid

#44646

Depositor

Kurt Thorn

Insert

yECitrine

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pAT222

Plasmid

#40942

Depositor

Angela Taddei

Article

Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

Insert

GFP-LacI**

Use

Tags

GFP(S65T, V163A) and SV40 NLS

Expression

Yeast

Mutation

LacI**/LacI2G(4 mutations) (see comment below); l…

Promoter

His3 promoter fragment

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pWS172

Plasmid

#90519

Purpose

Cas9 gap repair vector - HIS3

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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BB3cN_pGAP_23*_pLAT1_Cas9

Plasmid

#104906

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pWS174

Plasmid

#90961

Purpose

Cas9 gap repair vector - NatR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-3FLAG

Plasmid

#19337

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

3FLAG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pEDJ333

Plasmid

#177272

Purpose

GAL1p:Cas9:CYC1t

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Insert

Cas9

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GAL1

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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