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Plasmid#115698PurposeER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiaeDepositorInsertSSKar2-sfpHluorin-HDEL
UseTagsER retention signal containing HDEL and ER signal…ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#162606PurposePre-express Cas9 in yeastDepositorInsertTef1-Cas9
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL 103Q+ProGFPp416
Plasmid#15583DepositorInserthtt103Q + Pro (HTT Human)
UseTagsGFPExpressionYeastMutationProline-rich region presentPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-His-tag-HIS3MX6
Plasmid#20762DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHES830
Plasmid#87944PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human, Synthetic)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38
Plasmid#65516PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edCit-attR1 and attR2-edCer-cMycExpressionYeastMutationPromoterpPMA1Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM73
Plasmid#133651PurposeIntegration of CHS5-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-mCherry (CHS5 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-V5-hphMX4
Plasmid#20779DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only