8,390 results

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p415TEF cyto roGFP(iL)-Grx1(human)

Plasmid

#65002

Purpose

Yeast expression plasmid for roGFP1(iL)-Grx1; yeast codon optimized roGFP; human Grx1

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP(iL)

Expression

Yeast

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh1-PH

Plasmid

#71372

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa267-388

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
LD116

Plasmid

#69168

Purpose

Integration of GFP tag at Atg16 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg16-GFP

Tags

EGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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LD229

Plasmid

#69192

Purpose

Integration of 2GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg29-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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LD221

Plasmid

#69189

Purpose

Integration of 2GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg21-2EGFP

Tags

2xEGFP

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP38

Plasmid

#65516

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edCit-attR1 and attR2-edCer-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pRG211

Plasmid

#64519

Purpose

Centromeric E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDRFLIP34

Plasmid

#65512

Purpose

FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc

Expression

Yeast

Promoter

pPMA1

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK096

Plasmid

#65203

Purpose

Pre-Assembled Ura3 integration vector to be used in the Dueber YTK system.

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pKT-mCitrineV163A-HIS

Plasmid

#64685

Purpose

pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

mCitrine V163A

Expression

Yeast

Mutation

Valine 163 changed to alanine

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pYC61

Plasmid

#63925

Purpose

Recipient replicative vector to construct CFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

CFP

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pYC55

Plasmid

#63922

Purpose

Recipient replicative vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pYC42

Plasmid

#63920

Purpose

Recipient replicative vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

mCherry

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-PmCDA1

Plasmid

#60818

Purpose

to overexpress lamprey deaminase in yeast

Depositor

Youri Pavlov

Article

Lada et al PLoS Genet. 2013;9(9):e1003736. doi: 10.

Insert

CDA1

Tags

FLAG

Expression

Yeast

Promoter

GAL10

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRS424-TUB2

Plasmid

#60388

Purpose

Expression of yeast beta tubulin (TUB2) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pRS426-TUB1-internal6xHis

Plasmid

#60390

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4)

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
PhyB-mCherry-MYO1

Plasmid

#51570

Purpose

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

MYO1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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p41Neo MagicFluor

Plasmid

#58564

Purpose

p41Neo carrying both mating type markers buffered by the Cyc1 terminator

Depositor

Leonid Kruglyak

Insert

Ste2p-Citrine, Cyc1 term, Ste3p-mCherry

Expression

Yeast

Available Since

Nov. 24, 2014

Availability

Academic Institutions and Nonprofits only

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HC080

Plasmid

#59545

Purpose

Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag

Depositor

Daniel Klionsky

Insert

ATG13 (ATG13 Budding Yeast)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

AvrII site created after start codon

Promoter

ATG13

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only