9,747 results

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pUC18-GFP

Plasmid

#220495

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Publication

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

EGFP

Expression

Mammalian

Promoter

SV40

Available since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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V5-BS2-NIK3x (Nucleolus)

Plasmid

#220346

Purpose

Mammalian expression vector BS2 in nucleolus

Depositor

Bryan Dickinson

Publication

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

Nik3x (Nucleolus localization tag) and V5 tag

Expression

Mammalian

Promoter

CMV

Available since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-MBP-TEV-GG

Plasmid

#215800

Purpose

Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_FBXO3:1-471

Plasmid

#211071

Purpose

Mammalian expression of full-length human FBXO3. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXO3:M1-F471 (FBXO3 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-oB ONE-GO

Plasmid

#189733

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oB ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204817

Purpose

Bacterial expresison of Enterovirus D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV D68 3C protease

Expression

Bacterial

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-moxGFP-LMNB1

Plasmid

#207773

Purpose

Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

LMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-V5-Puro

Plasmid

#207730

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Zeo

Plasmid

#207733

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-moxGFP-Puro

Plasmid

#207721

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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qTAG-N-Puro-3xHA

Plasmid

#207696

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAM/SAR-CAG-aSyn(A53T)/HA-WPRE-bGHpA

Plasmid

#194242

Purpose

Expresses human alpha-Synuclein A53T

Depositor

Michael J Fox Foundation MJFF

Insert

aSynuclein(A53T) (SNCA Human)

Use

AAV

Tags

HA

Mutation

A53T

Promoter

SAR-CAG

Available since

Jan. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HB

Plasmid

#184868

Purpose

pSwap plasmid part with HB homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pABVec-NbH11-H4_huIgG1

Plasmid

#160313

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Publication

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH H4-Fc

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pABVec-NbH11-D4_huIgG1

Plasmid

#160314

Purpose

Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2 as a huIgG1 Fc fusion

Depositor

Ray Owens

Publication

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

VHH D4-Fc

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcI-ts,ind+-(mtSSB delta20)

Plasmid

#186280

Purpose

Expresses Human Mitochondrial SSB in E. coli

Depositor

Kenneth Johnson

Publication

Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

Insert

Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)

Expression

Bacterial

Mutation

First 20 amino acids corresponding to probable mi…

Promoter

lambda

Available since

July 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS SARS-CoV-2 Alpha Spike

Plasmid

#185691

Purpose

Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

Depositor

Marceline Côté

Publication

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Alpha Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: Δ69-70, Δ145, N…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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