9,747 results

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pKanB

Plasmid

#223523

Purpose

Used for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Publication

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Publication

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1

Plasmid

#223718

Purpose

Expresses FLAG-TurboID-mutLIR-SQSTM1

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Mutation 335-DDDW-338 to 335-AAAA-338

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp3-c3xFLAG

Plasmid

#215696

Purpose

Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Publication

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp3

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Publication

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-Flag-CIP2A

Plasmid

#222623

Purpose

mammalian expression vector of Flag-tagged CIP2A

Depositor

Daniel Durocher

Publication

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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V5-BS2-SENP2 (Nuclear Pore)

Plasmid

#220345

Purpose

Mammalian expression vector BS2 in nuclear pore

Depositor

Bryan Dickinson

Publication

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

SENP2 (nuclear pore localized protein) and V5 tag

Expression

Mammalian

Promoter

CMV

Available since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGTM_COV2_NSP5_C145A_001_SUMO

Plasmid

#192349

Purpose

Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

Depositor

Gaetano Montelione

Publication

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Mutation

Cysteine 145 of the active site is substituted wi…

Promoter

T7 lac

Available since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Publication

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-GST-TEV-GG

Plasmid

#215795

Purpose

Cloning of target gene with N terminal GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-HIS6-NUSA-TEV-GG

Plasmid

#215798

Purpose

Cloning of target gene with N terminal His6 NUSA tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-CT-sfGFP-HN-GG

Plasmid

#215803

Purpose

Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNIC-CTH10STII-GG

Plasmid

#215804

Purpose

Cloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tag

Depositor

ASAP Discovery, Frank von Delft

Publication

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Fusion of Zika NS2B-NS3 proteins

Plasmid

#204815

Purpose

Bacterial expression of a fusion of Zika NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika NS2B-NS3 fusion (orf1ab Zika)

Expression

Bacterial

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-LASV-GPC

Plasmid

#215690

Purpose

Produces the Lassa virus glycoprotein

Depositor

Melinda Brindley

Publication

Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

Insert

LASV GP

Expression

Mammalian

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_RAB7A:1-207

Plasmid

#211096

Purpose

Mammalian expression of full-length human RAB7A. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_BOP1:1-746

Plasmid

#211005

Purpose

Mammalian expression of full-length humanBOP1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

BOP1:M1-T746 (BOP1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_WDR91:1-747

Plasmid

#210997

Purpose

Mammalian expression of full-length human WDR91. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_DCAF1:1-1507

Plasmid

#210972

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-i1 ONE-GO

Plasmid

#204333

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Publication

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i1 ONE-GO

Use

Lentiviral

Available since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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