Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

9,746 results

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pDONR221-SLC35A2_STOP

Plasmid

#161313

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35A2 (SLC35A2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC30A7_STOP

Plasmid

#161314

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC30A7 (SLC30A7 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC39A6_STOP

Plasmid

#161319

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A6 (SLC39A6 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC38A3_STOP

Plasmid

#161308

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A3 (SLC38A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC29A3_STOP

Plasmid

#161293

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC29A3 (SLC29A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC2A1_STOP

Plasmid

#161269

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A1 (SLC2A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC39A14_STOP

Plasmid

#161271

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A14 (SLC39A14 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC35G6_STOP

Plasmid

#161262

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC35G6 (SLC35G6 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-HSlig3

Plasmid

#165574

Purpose

Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

DNA Ligase III

Use

Synthetic Biology

Available Since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-T4 PGD

Plasmid

#165550

Purpose

T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T4 PGD (T4 Endonuclease V)

Use

Synthetic Biology

Available Since

Aug. 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-PstIM

Plasmid

#165536

Purpose

Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.PstI

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-SfiIR (cDNA)

Plasmid

#165528

Purpose

Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

SfiI (used to create cDNA libraries)

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-Eco31IB

Plasmid

#165527

Purpose

Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Eco31IB

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-NcoIM

Plasmid

#165578

Purpose

Site-specific DNA methyltransferase; protects DNA from cleavage by NcoI

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.NcoI

Use

Synthetic Biology

Available Since

Aug. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-Eco31IA

Plasmid

#165517

Purpose

Site-specific DNA methyltransferase 1; protects DNA from cleavage by Eco31I

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Eco31IA

Use

Synthetic Biology

Available Since

July 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC26A6_STOP

Plasmid

#161222

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A6 (SLC26A6 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A44_STOP

Plasmid

#161208

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A44 (SLC25A44 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC24A1_STOP

Plasmid

#161215

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC24A1 (SLC24A1 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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