Addgene

Showing: 1 - 10 of 63 results

Results per page:

Sort results by:

cPE

Plasmid

#183549

Purpose

Expresses compact Prime Editor in mammalian cells

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

SpCas9 nickase (H840A), Reverse Transcriptase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CPEB1

Plasmid

#155472

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPEB1 (CPEB1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad33_ISCpe2_reRNA

Plasmid

#205335

Purpose

Bacterial expression of ISCpe2 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCpe2 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-niCPE

Plasmid

#213747

Purpose

For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-nLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A in LbCas12a

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U1A

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV8-cPE-N

Plasmid

#183538

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

N-terminal fragment of SpCas9 nickase (H840A)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-nuCPE

Plasmid

#213748

Purpose

For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-LbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R in LbCas12a

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS cPea3 (CT#1024)

Plasmid

#13906

Depositor

Cliff Tabin

Article

Brent et al Development. 2004 Aug . 131(16):3885-96.

Insert

Pea3 (ETV4 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
TetO7-HSP30UTR-nLucPEST-MS2(v4)

Plasmid

#199764

Purpose

Control construct of elongation reporter to quantify elongation of LacZ

Depositor

Brian Zid

Article

Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

Insert

NanolucPEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge