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Showing: 1 - 50 of 653 results

  1. pGEX4-4T-1 Pontibacillus AGSC

    Plasmid
    #161814
    Purpose
    Expresses AGSC domain from Pontibacillus A/G cyclase enzyme
    Depositor
    Douglas Feldman
    Insert
    AGSC domain
    Use
    Tags
    His and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161814-plasmid-map-sequence-id-319428

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  2. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  3. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  15. pJK040 (pET-PurQ-A128T)

    Plasmid
    #73840
    Purpose
    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Article
    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A128T mutant
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    73840-plasmid-map-sequence-id-136936

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  16. pJK034

    Plasmid
    #72383
    Purpose
    Produces Bacillus subtilis glycinamide ribonucleotide synthetase (PurD)
    Depositor
    T. J. Kappock, JoAnne Stubbe
    Insert
    phosphoribosylglycinamide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72383-plasmid-map-sequence-id-136200

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  17. pJK100

    Plasmid
    #73264
    Purpose
    Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylformylglycinamidine synthase subunit PurS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73264-plasmid-map-sequence-id-134799

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  31. pGL003

    Plasmid
    #215335
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215335-plasmid-map-sequence-id-426095

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  32. pGL004

    Plasmid
    #215336
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)
    Depositor
    Gene-Wei Li
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hyper-pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215336-plasmid-map-sequence-id-426101

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  33. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

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  34. pCG-A-sacB(SP)-B

    Plasmid
    #87370
    Purpose
    An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    SacB signal peptide
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87370-plasmid-map-sequence-id-183403

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  35. pCG-A-celA(SP)-B

    Plasmid
    #87371
    Purpose
    An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    CelA signal peptide
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87371-plasmid-map-sequence-id-183393

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  36. pCG-A-xynA(SP)-B

    Plasmid
    #87372
    Purpose
    An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly
    Depositor
    Tom Ellis
    Article
    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021
    Insert
    XynA signal peptide
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87372-plasmid-map-sequence-id-183392

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  45. pGEX-GST-BcPC-PLC

    Plasmid
    #224255
    Purpose
    Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli
    Depositor
    Chiaki Murakami
    Article
    Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:
    Insert
    Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
    Use
    Tags
    GST and HRV-3C (LEVLFQGP) site
    Expression
    Bacterial
    Mutation
    The gene of Bacillus cereus ATCC 14579 was used f…
    Promoter
    lac promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    224255-plasmid-map-sequence-id-457146

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  50. pMT1004

    Plasmid
    #8613
    Depositor
    Robert Hartley
    Insert
    binase, barstar
    Use
    Tags
    Expression
    Bacterial
    Mutation
    as pMT1002
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    8613-plasmid-map-sequence-id-240677

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Showing: 1 - 50 of 653 results