Skip to main content
Addgene
Showing: 1 - 25 of 25 results

  1. PumA

    Plasmid
    #87319
    Purpose
    PumHD w/each unit mutated to bind A (as in Fig. 1C; unit 6 is the Pumby for A)
    Depositor
    Edward Boyden
    Article
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Insert
    Pumilio protein mutant (PUM1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    all 8 units mutated to bind A
    Promoter
    UBC
    Available since
    June 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    87319-plasmid-map-sequence-id-181378

    Enlarge

  8. Venus-Puma-4E-pEGFP-C1

    Plasmid
    #166746
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Puma-4E (BBC3 Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166746-plasmid-map-sequence-id-329007

    Enlarge

  14. HCA2-DuET

    Plasmid
    #213308
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213308-plasmid-map-sequence-id-430846

    Enlarge

  15. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid
    #196974
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag
    Depositor
    Lukas Kapitein
    Article
    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Use
    Tags
    GST and mNeonGreen
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    196974-plasmid-map-sequence-id-388923

    Enlarge

  17. HCA3-Tango

    Plasmid
    #66397
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HCA3 (HCAR3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66397-plasmid-map-sequence-id-263507

    Enlarge

  19. HCA3-DuET

    Plasmid
    #213309
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HCA3 (HCAR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213309-plasmid-map-sequence-id-430847

    Enlarge

  20. HCA2-Tango

    Plasmid
    #66396
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HCA2 (HCAR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66396-plasmid-map-sequence-id-372748

    Enlarge

  21. pCiES

    Plasmid
    #60010
    Purpose
    Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.
    Depositor
    David Russell
    Article
    Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.
    Insert
    Env (foamy virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    60010-plasmid-map-sequence-id-211473

    Enlarge

  23. pCiPS-NIFV

    Plasmid
    #110807
    Purpose
    Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    Pol
    Use
    Tags
    Expression
    Mammalian
    Mutation
    2 mutations in the DDE catalyic core motif of the…
    Promoter
    CMV
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110807-plasmid-map-sequence-id-222708

    Enlarge

  24. pNIFV

    Plasmid
    #111085
    Purpose
    pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
    Use
    Deleted foamy virus
    Tags
    Expression
    Mutation
    at nt 2334 A changed to T, at nt 2495 A changed t…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111085-plasmid-map-sequence-id-212298

    Enlarge

Showing: 1 - 25 of 25 results