We narrowed to 37 results for: spi1
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EF1a_SPI1_P2A_Hygro_Barcode
Plasmid#120482PurposeBarcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPI1_pet28a
Plasmid#131653PurposeBacterial expression of ETS gene SPI1DepositorInsertSPI1 (SPI1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-SPI1
Plasmid#97039PurposeExpresses SPI1 in mammalian cellsDepositorInsertSpi-1 Proto-Oncogene (SPI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SPI1
Plasmid#179514PurposeTunable and temporal expression control of SPI1DepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GS PromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SPI1 P2A mCherry
Plasmid#140029PurposeTunable and temporal expression control of SPI1 and mCherryDepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SPI1 P2A mCherry
Plasmid#140031PurposeTunable and temporal expression control of SPI1 and mCherryDepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag Pu.1
Plasmid#66974PurposeExpression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of proteinDepositorInsertSpi1 (Spi1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV PIG Pu.1
Plasmid#66982PurposeRetroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFPDepositorInsertSpi1 (Spi1 Mouse)
UseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
1kb Zfp521 promoter reporter
Plasmid#104188Purposeluciferase reporter to assay Zfp521 promoter activationDepositorInsertZfp521 promoter - 1 Kb (Zfp521 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60-mPU.1-ETS
Plasmid#85733PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4DepositorInsertETS domain of transcription factor PU.1 (Spi1 Mouse)
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Pu.1
Plasmid#139839Purposedoxycycline-inducible expression of mouse Pu.1 in mammalian cellsDepositorInsertPu.1 (Spi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorInsertspi1 (SPI1 Human)
UseTagsNoneExpressionBacterialMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-PU.1_DBD(168-270)
Plasmid#188053PurposeExpresses His8-PU.1_DBD(168-270) with a TEV site for removal of the His8 tag.DepositorInsertSPI1 (SPI1 Human)
UseTagsHis8ExpressionBacterialMutationdeleted amino acids 1-167PromoterT7Available sinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF1652
Plasmid#143532PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1653
Plasmid#143533PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSPI1 (SPI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS
Plasmid#85734PurposeExpresses DNA-binding (ETS) domain of murine PU.1, residues 167-272DepositorInsertETS domain of murine PU.1 (residues 167-272) (Spi1 Mouse, Human)
UseTags6xHis following thrombin cleavage site LVPR|GExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PU.1-IRES-mCherry
Plasmid#80140Purposeretroviral expression of PU.1 and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZRS PU.1 WT
Plasmid#34835DepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationPromoterLTRAvailable sinceJan. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
PU.1-W215G LZRS
Plasmid#44635DepositorInsertPU.1 W215G (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutation215 mutation (W to G)PromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
PU.1-S148A LZRS
Plasmid#44633DepositorInsertPU.1 S148A (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutation148 mutation (S to A)PromoterAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
PU.1-delta75-100 LZRS
Plasmid#44634DepositorInsertPU.1 Δ75-100 (Spi1 Mouse)
UseRetroviralTagsEGFP and IRESExpressionMammalianMutationdeletion of aa 75-100PromoterAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS
Plasmid#124652PurposeChimeric ETS domainDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmiReport PU.1 3' UTR
Plasmid#26530DepositorInsertPU.1 3' UTR (Spi1 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
PU.1-ets-IRES-mCherry
Plasmid#80141Purposeretroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherryDepositorInsertPU.1 (Spi1 Mouse)
UseRetroviralTagsIRES-mCherryExpressionMammalianMutationets = PU.1 DNA binding domain (amino acids 159-26…PromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3
Plasmid#124435PurposeChimeric ETS domain: H3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis TagExpressionBacterialMutationResidues 225 to 239 replaced with corresponding s…PromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Loop
Plasmid#124551PurposeChimeric ETS domain: Loop of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 216 to 223 replaced with corresponding s…PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236H
Plasmid#124616PurposeChimeric ETS domain: N236HDepositorUseTagsExpressionMammalianMutationResidues 236 altered from asparagine to a histidi…PromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236Q
Plasmid#124615PurposeChimeric ETS domain: N236QDepositorUseTagsExpressionMammalianMutationResidues 236 altered from asparagine to a glutami…PromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PU.1-ETS N236Y
Plasmid#124614PurposeChimeric ETS domain: N236YDepositorUseTagsExpressionMammalianMutationResidues 236 altered from asparagine to a TyrosinePromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-S3
Plasmid#124550PurposeChimeric ETS domain: S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 242 to 246 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-PU.1-ETS-H2
Plasmid#123358PurposeChimeric ETS domain: H2 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 207 to 215 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-H3/S3
Plasmid#124627PurposeChimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationResidues 237 to 241 replaced with corresponding s…PromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only
