We narrowed to 1,606 results for: CAST
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Plasmid#236111PurposeEncodes sfGFP BbsI dropout for cloning in guide RNA sequence, encodes mScarlet BsmBI/BsaI dropout for cloning in cargo sequenceDepositorTypeEmpty backboneAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only
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pRhaCAST
Plasmid#211791PurposepZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoterDepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
UseCRISPRExpressionBacterialPromoterRhamnose-inducibleAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTarget_CAST
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN7CAST
Plasmid#190661PurposeExpresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRESDepositorInserthuman codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
ExpressionMammalianPromoterCMVAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonor_AcCAST_kanR
Plasmid#127925PurposeKanamycin resistance donor for AcCASTDepositorInsertKanR
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AcCAST
Plasmid#127923PurposeExpresses AcCAST and a crRNA targeting PSP1DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST
Plasmid#127922PurposeExpresses ShCAST and a crRNA targeting PSP1DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MultiCAST-Helper
Plasmid#248973PurposeMultiCAST helper plasmid encoding CAST Tn6677 CRISPR and transposition proteinsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCgVchCAST-crtYf
Plasmid#203816PurposeExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
UseCRISPRExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCascade_SPS1(PmcCAST)
Plasmid#224926PurposeExpression and purification of type I-B2 Cascade in CRISPR-associated transposon of RomsettaDepositorInsertsCas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression
UseCRISPRExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST-pyrE
Plasmid#203813PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET_StrepII_TEV_LIC_TniQ(PmcCAST)
Plasmid#224927PurposeTniQ expression plasmid for the co-purification of TnsC-TniQ complexDepositorInsertTniQ
UseCRISPRExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCaST-dVP16AD
Plasmid#15306DepositorInsertdVP16AD-ZIP+
ExpressionInsectAvailable SinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
CASTOR1-HA (D304A)
Plasmid#237419PurposeTransient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)DepositorAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
MultiCAST-miniTn-Kan
Plasmid#248974PurposeMultiCAST mini-Tn donor plasmid containing kanamycin selectable markerDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 10, 2026AvailabilityAcademic Institutions and Nonprofits only