We narrowed to 296 results for: actb

1. pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB

   Plasmid

   #169921

   Purpose

   DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.

   Depositor

   Zhili Rong

   Article

   Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105

   Insert

   IRES-Puro-pEF1a-EGFP
   

   Use

   CRISPR

   Promoter

   EF1a

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ACTB-(A)4x

   Plasmid

   #112058

   Purpose

   ACTB mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACTB-(A)1x

   Plasmid

   #112055

   Purpose

   ACTB mRNA tagged with 1 copy of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   1 copy of Riboglow tag (variant A) after stop cod…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. ACTB-(A)3x

   Plasmid

   #112057

   Purpose

   ACTB mRNA tagged with 3 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   3 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ACTB-(A)2x

   Plasmid

   #112056

   Purpose

   ACTB mRNA tagged with 2 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   2 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available Since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458 ACTB

   Plasmid

   #229975

   Purpose

   ecnodes for spCas9 and sgRNA targeting last intron of ActinB gene

   Depositor

   Martin Pelosse

   Article

   Pelosse et al Synthetic Biology 2025.02.13

   Insert

   ActBsgRNA and spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUB_smFLAG_ActB_MS2

   Plasmid

   #81083

   Purpose

   Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_ActB_24xMS2
   

   Expression

   Mammalian

   Available Since

   Aug. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. LACTB2

   Plasmid

   #39115

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   LACTB2 (LACTB2 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pKlf4_Halo_Ty2_BactBsd

   Plasmid

   #235512

   Purpose

   C-term HALO targeting construct

   Depositor

   Brian Hendrich

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Mouse Targeting

   Tags

   HALO, 2xTy

   Mutation

   None

   Promoter

   beta actin

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS_GGSG_mAID_HA_BactBsd

    Plasmid

    #235504

    Purpose

    single mAID_3xHA cloning vector with Roxed Bact-Bsd

    Depositor

    Brian Hendrich

    Insert

    mini-Auxin Inducible Degron
    

    Use

    Mouse Targeting

    Tags

    mAID, HA

    Mutation

    None

    Promoter

    beta actin

    Available Since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSox2_Halo_Ty2_BactBsd

    Plasmid

    #235513

    Purpose

    C-term HALO targeting construct

    Depositor

    Brian Hendrich

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Mouse Targeting

    Tags

    HALO, 2xTy

    Mutation

    None

    Promoter

    beta actin

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. cACTB

    Plasmid

    #236544

    Purpose

    For expression of ACTB in mammalian cells

    Depositor

    Sandra Citi

    Article

    Mauperin et al Nat Commun. 2025 Mar 13;16(1):2514. doi:

    Insert

    ACTB
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hpACTB

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. p140Tol2-Olactb:SABER

    Plasmid

    #226829

    Purpose

    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    BFP-SABER
    mCherry-t2A-CreERT2
    

    Use

    Zebrafish expression

    Promoter

    Olactb and hsp70

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiCRISPRv2-ACTB-C1

    Plasmid

    #169796

    Purpose

    Expresses Cas9 and guide RNA for ACTB gene in human

    Depositor

    Natsuko Chiba

    Article

    Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_Actb HR donor

    Plasmid

    #97317

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97309

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPRv2-ACTB-C4

    Plasmid

    #229849

    Purpose

    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. psBIG1a ActBT2ATetP2AP - H

    Plasmid

    #229972

    Purpose

    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    ActBT2ATetP2AP - H
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCG-ACTB-C4

    Plasmid

    #229846

    Purpose

    Expresses wild-type Cas9 and gRNA for ACTB gene.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA for ACTB gene
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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