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ZE11 tiggywinkle hedgehog CMV

Plasmid

#17060

Depositor

Randall Moon

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE10 sonic hedgehog pT7TS

Plasmid

#17059

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

sonic hedgehog (shha Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE12 sonic hedgehog CMV

Plasmid

#17061

Depositor

Randall Moon

Insert

sonic hedgehog (shha Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE9 tiggywinkle hedgehog pT7TS

Plasmid

#17058

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
MS03

Plasmid

#164526

Purpose

Expresses Sonic Hedgehog

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Sonic Hedgehog (SHH Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS (A) shh (CT#251)

Plasmid

#13991

Depositor

Cliff Tabin

Article

Riddle et al Cell. 1993 Dec 31. 75(7):1401-16.

Insert

shh (SHH Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (E) shh (CT#489)

Plasmid

#13992

Depositor

Cliff Tabin

Article

Riddle et al Cell. 1993 Dec 31. 75(7):1401-16.

Insert

shh (SHH Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
EF.hICN1

Plasmid

#113715

Purpose

Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

None

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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XE13 Xbhh

Plasmid

#16993

Depositor

Randall Moon

Article

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh (ihh Frog)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pGL4.R_8xGLIBS_minPro_Luc2p_Ren

Plasmid

#249281

Purpose

Dual-luciferase GLI reporter plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Puro 8xGLI_minPro_Luc2p_Ren

Plasmid

#249282

Purpose

Dual-luciferase GLI reporter viral plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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M16 pCS-twhh-bGFP

Plasmid

#17153

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

bGFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
XE15 Xbhh-C

Plasmid

#16995

Depositor

Randall Moon

Article

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh-C (ihh Frog)

Use

Xenopus expression

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
XE14 Xbhh-N

Plasmid

#16994

Depositor

Randall Moon

Article

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh-N (ihh Frog)

Use

Xenopus expression

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
M15 pCS-twhh-nBeta gal

Plasmid

#17152

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

nuclear beta-gal

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
M18 pCS-twhh-nBeta gal Vec

Plasmid

#17155

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

nuclear beta-gal

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only