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pBabe K-Ras 12V

Plasmid

#12544

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

K-Ras 12V (KRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

12V constituitively activated

Promoter

Available since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

Plasmid

#120562

Purpose

Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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R979-X58-008: Hs.KRAS4b(1-188)

Plasmid

#159691

Purpose

Baculovirus protein expression of KRAS4b (1-188)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-188) (KRAS Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

Plasmid

#120561

Purpose

Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-MBP-FRB-KRas/N12

Plasmid

#214302

Purpose

Express split-KRas fragment fused with FRB and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FRB (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Promoter

Tac

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b-KRas/13C-FKBP-MBP

Plasmid

#214303

Purpose

Express split-KRas fragment fused with FKBP and MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Split-KRas fragment fused with FKBP (KRAS Human)

Use

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

KRas-Q61L

Promoter

T7

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0541

Plasmid

#142276

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GCFC2 (C2orf3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0540

Plasmid

#142275

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GCFC2 (C2orf3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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R979-X57-008: Hs.KRAS4b(1-169)

Plasmid

#159690

Purpose

Baculovirus protein expression of KRAS4b (1-169)

Depositor

Dominic Esposito

Article

Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

Insert

Hs.KRAS4b(1-169) (KRAS Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS

Plasmid

#111849

Purpose

Expresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS (KRAS Human)

Use

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

G12C

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(WT)

Plasmid

#224283

Purpose

Cell transfection and expression of K-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro shKRAS

Plasmid

#116871

Purpose

Tet-inducible suppression of KRAS

Depositor

William Hahn

Article

Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

Insert

shKRAS (KRAS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1/TO

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pInducer10b-EGFP-KRAS-WT

Plasmid

#164935

Purpose

Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

Depositor

Ji Luo

Article

Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

Inserts

Enhanced green fluorescent protein
KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ubc Promoter and Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pJV136

Plasmid

#124255

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 9

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode

Plasmid

#170237

Purpose

Barcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

KRAS (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-KRAS

Plasmid

#52729

Purpose

retroviral expression of human KRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJV132

Plasmid

#124254

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 3

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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