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We narrowed to 295 results for: pex2

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pFA6a-FLAG-APEX2-NES-K.l.LEU2

Plasmid

#229229

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-TRP1

Plasmid

#229228

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pERG6

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pOM14

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pCOX4

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GABARAPL2Y49A/L50A

Plasmid

#175752

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GABARAPL2Y49A/L50A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y49A L50A

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen

Plasmid

#218645

Purpose

Gateway shuttling vector for APEX2-tagged P4C, a P4C biosensor

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

SidC(P4C)

Use

Gateway entry

Tags

Expression

Mutation

Promoter

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

Plasmid

#182925

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Use

Tags

APEX2 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

Plasmid

#187588

Purpose

Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NLS

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

Plasmid

#187582

Purpose

Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SRSF7 (SRSF7 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Use

Tags

APEX2 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

Plasmid

#187583

Purpose

Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

PML (PML Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-APEX2-Ires-eGFP-PAC

Plasmid

#180142

Purpose

used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

Plasmid

#187584

Purpose

Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

SP100 (SP100 Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

Plasmid

#187585

Purpose

Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

NPAT (NPAT Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

Plasmid

#187576

Purpose

Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

LMNA (LMNA Human)

Use

Tags

APEX2-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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