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Plasmid#196218PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV CAG.FLEX iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt
Plasmid#175184PurposeFluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPIDepositorInsertpAAV hSyn FLEX iGluSnFR3 v82.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI Anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.iGluSnFR3.v857.SGZ
Plasmid#178337PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV GFAP iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt
Plasmid#175180PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn FLEX iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
Plasmid#175182PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.FLEX.iGluSnFR3.v857.PDGFR
Plasmid#196219PurposeFluorescent reporter for glutamate, third generation, variant 857 in PDGFR backbone. iGluSnFR3.v857.PDGFRDepositorInsertpAAV CAG FLEX iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI
Plasmid#196223PurposeFluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPIDepositorInsertpAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsGPI anchor, IgK-chain, and Myc epi tagExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR
Plasmid#196226PurposeFluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGRDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.NGR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and NGR TM DomainExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-Ns4A-tv-HCVp-P3
Plasmid#179674PurposeTVMVP-repressible ER-associated HCVPDepositorInsertHCVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-P3-cTVMVP
Plasmid#179664PurposeER-associated uncaged-cTVMVP with P3 leucine zipperDepositorInsertcTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-FKBP-nTEVP
Plasmid#179675PurposeER-associated FKBP split nTEVP (Rapalog inducible)DepositorInsertFKBP-nTEVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-nTVMVp-AP4
Plasmid#179669PurposeER-associated uncaged-nTVMVP with AP4 leucine zipperDepositorInsertnTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-nTVMVp-hcvs-AP4
Plasmid#179673PurposeHCVP-repressible ER-associated uncaged-nTVMVPDepositorInsertnTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-SpyCatcher
Plasmid#179677PurposeHCVP-cleavable ER-associated SpyCatcherDepositorInsertHCVP SpyCatcher
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gβγ-BERKY1
Plasmid#158429PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK3ct
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY3
Plasmid#158428PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseLentiviralTagsMycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorInsertEGFP
UseTagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
NCVB-ssGrl1-ESCargo*
Plasmid#140167PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Tetrahymena thermophilaDepositorInsertssGrl1-DsRed-Express2-FKBP(LV)(C22V)
UseOtherTagsExpressionMutationPromoterMTT1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-mCherry
Plasmid#65301Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_puromycin
Plasmid#65309PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
UseTagsExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-FRB-cTEVP
Plasmid#179676PurposeER-associated FRB split-cTEVP (Rapalog inducible)DepositorInsertFRB (MTOR Human, Synthetic)
UseTagscTEVPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-mCherry-Ecadherin
Plasmid#65289Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only