We narrowed to 584 results for: pcmv gfp

1. pCMV-T7-BPNLS-CE-ABE8e-SpRY--BPNLS-P2A-EGFP (NK289)

   Plasmid

   #208293

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpRY A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpRY(D10A/A6…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

   Plasmid

   #185490

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

   Plasmid

   #208292

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA inlaid into nSpCas9(D10A)

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

   Plasmid

   #185489

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

   Plasmid

   #185492

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

   Plasmid

   #221081

   Purpose

   SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

   Plasmid

   #176461

   Purpose

   Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid

    #185488

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid

    #185485

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid

    #208290

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpCas9(D10…

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)

    Plasmid

    #185484

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)

    Plasmid

    #185486

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)

    Plasmid

    #185487

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-P2A-eGFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176459

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid

    #176460

    Purpose

    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

    Plasmid

    #210516

    Purpose

    expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

    Plasmid

    #210505

    Purpose

    expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV6-AC 4-μHOM

    Plasmid

    #113619

    Purpose

    Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    4-μHOM variant of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-dCas12a-BE

    Plasmid

    #193648

    Purpose

    Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-rAPOBEC1, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-monSTIM1

    Plasmid

    #161795

    Purpose

    Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

    Insert

    MonSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-tomm20-ECFP-RspANFAST

    Plasmid

    #216348

    Purpose

    targets ECFP-RspANFAST to mitochondrial membrane

    Depositor

    Alison Tebo

    Insert

    tomm20-ECFP-RspANFAST
    

    Use

    Tags

    tomm20

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-PE2-P2A-PuroR

    Plasmid

    #196962

    Purpose

    Expresses PE2 with puromycin resistance

    Depositor

    Leopold Parts

    Insert

    PE2-P2A-Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-160

    Plasmid

    #79840

    Purpose

    Expresses NLS-PpsR2-mVenus under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-mVenus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-iGlu3Fast

    Plasmid

    #226500

    Purpose

    Ultrafast-decay iGluSnFR3 variant for tracking high frequency synaptic glutamate release

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 S72T
    

    Use

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    S72T

    Promoter

    CMV

    Available since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-GCaMP6fu

    Plasmid

    #86235

    Purpose

    Express fluorescent calcium indicator GCaMP6fu in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

    Insert

    GCaMP6fu
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W43Y D395A

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-iGlu3slow1

    Plasmid

    #226501

    Purpose

    Superslow-decay iGluSnFR3 variant 1 for population imaging

    Depositor

    Katalin Torok

    Article

    Tran et al bioRxiv, August 30th, 2024

    Insert

    iGluSnFR3 D25A
    

    Use

    Tags

    IgK, Myc tag, and PDGFR Beta TM domain

    Expression

    Mammalian

    Mutation

    D25A

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge