Addgene

We narrowed to 812 results for: PC1;

Showing: 181 - 200 of 812 results

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pcDNA3-hPC1_MTS_L4137P-eGFP

Plasmid

#108403

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS-eGFP

Plasmid

#108402

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-mito-DAAO

Plasmid

#141132

Purpose

Expresses DAAO with mitochondrial localization signal in mammalian cells

Depositor

Vsevolod Belousov

Insert

mito-DAAO

Use

Tags

2 mitochondrial localization signals and 7xHis

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX NPC1-FLAG-TiD

Plasmid

#199748

Purpose

Lentiviral construct to stably express NPC1 TurboID

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

NPC1 (NPC1 Human)

Use

Tags

Flag and TurboID

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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yWL234 MCP-GFP-PABPC1*

Plasmid

#197054

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-PABPC1*

Depositor

Robert Singer

Article

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-PABPC1*

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

ubc

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red-C199S

Plasmid

#48252

Purpose

Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED-C199S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
PC15-421-14-1_TRIM24-BD-VPR

Plasmid

#200435

Purpose

Expresses the VPR transactivation domain linked to the TRIM24(BD) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

TRIM24(BD)-VPR

Use

Tags

TRIM24(BD), VPR

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(P691S)-FLAG

Plasmid

#164973

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

P691S

Promoter

EF1a

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(F503/4A)-FLAG

Plasmid

#164974

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

F503A and F504A

Promoter

EF1a

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pC1 Grx1-roCherry-mito

Plasmid

#141069

Purpose

Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

Depositor

Dmitry Bilan

Article

Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

Insert

Grx1-roCherry

Use

Tags

dMTS

Expression

Mammalian

Mutation

Changed Alanine 150 to Cysteine, inserted Threoni…

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1 MLLEmut

Plasmid

#65808

Purpose

expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC2 Human)

Use

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

MLLEmut

Promoter

CMV

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-eGFP-PC1-3HA

Plasmid

#108406

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

3HA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(D786N)-FLAG

Plasmid

#164975

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D786N, insertion L aa15 (please see depositor co…

Promoter

EF1a

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_4106-eGFP-3HA

Plasmid

#108399

Purpose

Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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TRAPPC10_pLENTI-CAG-IRES-GFP

Plasmid

#176998

Purpose

Mammalian lentiviral expression vector encoding TRAPPC10

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

TRAPPC10 (TRAPPC10 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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