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pmGFP-P2A-K(AAA)12-P2A-RFP

Plasmid

#105687

Purpose

Double fluorescent stalling reporter K(AAA)12

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

Plasmid

#127310

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

SV40

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

Plasmid

#131256

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains point mutations that convert USP7 aspart…

Promoter

Availability

Academic Institutions and Nonprofits only

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(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

Plasmid

#160739

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Availability

Academic Institutions and Nonprofits only

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(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

Plasmid

#160740

Purpose

Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Availability

Academic Institutions and Nonprofits only

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Cenp-B DBD INCENP TSS/AAA mCherry

Plasmid

#45234

Purpose

Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry

Use

Tags

CENP-B DBD, DNA binding domain of CENP-B and mChe…

Expression

Mammalian

Mutation

INCENP C-terminal TSS mutated to AAA

Promoter

t7

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R AAA mutant

Plasmid

#13510

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Promoter

Availability

Academic Institutions and Nonprofits only
1129 pcDNA3 GFP FKHR H215R AAA

Plasmid

#9034

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Promoter

Availability

Academic Institutions and Nonprofits only
1130 pBabe puroL HA FKHR H215R AAA

Plasmid

#9035

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Promoter

Availability

Academic Institutions and Nonprofits only
1357 pcDNA3 flag FKHR AAA delta DB

Plasmid

#10696

Depositor

William Sellers

Insert

FKHR AAA deltaDB (FOXO1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

T24A S256A S319A , deletion in aa 208-220

Promoter

Availability

Academic Institutions and Nonprofits only
1358 pBabe puroL FKHR AAA delta DB

Plasmid

#10697

Depositor

William Sellers

Insert

FKHR AAA deltaDB (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A , deletion in aa 208-220

Promoter

Availability

Academic Institutions and Nonprofits only
pnEA-NpM-CteIF4G_996-1033-YLLtoAAA-GB1_AF

Plasmid

#148706

Purpose

Bacterial Expression of CteIF4G_966-1033-YLLtoAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

CteIF4G_966-1033-YLLtoAAA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X

Plasmid

#148000

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Availability

Academic Institutions and Nonprofits only

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(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

Plasmid

#168189

Purpose

HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-nUbc6E-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Availability

Academic Institutions and Nonprofits only

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(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

Plasmid

#168192

Purpose

HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Depositor

Christina Woo

Article

Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

Insert

HA-LaG6-(EAAAK)4-OGT(4)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Availability

Academic Institutions and Nonprofits only

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pMDC32-FIT2-FLL[157-159]AAA

Plasmid

#96991

Purpose

Express mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Use

Tags

Expression

Plant

Mutation

Amino acid residues 157-159 were mutated (FLL[157…

Promoter

35S

Availability

Academic Institutions and Nonprofits only

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pMDC43-FIT2-FLL[157-159]AAA

Plasmid

#96994

Purpose

Express GFP-tagged mutated mouse FIT2 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

Insert

MmFIT2 (Fitm2 Mouse)

Use

Tags

GFP

Expression

Plant

Mutation

Amino acid residues 157-159 were mutated (FLL[157…

Promoter

35S

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PEST

Plasmid

#127320

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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